PDF(Pubmed)
Abstract：
Basal cell carcinoma (BCC) is classified histologically into subtypes that determine treatment decisions. MicroRNAs (miRs) are short noncoding RNAs that may serve as diagnostic biomarkers. We investigated if particular miRs could distinguish BCC subtypes. We sequenced miRs from 55 archival BCC and 9 control skin specimens and then validated these miRs by qRT-PCR assay on a second BCC cohort (18 superficial, 16 nodular, 15 infiltrative) and control skin (n = 12). Expression values for individual miRs were normalized to miR-16-5p, which was the least variant among the control skin and BCC samples. We found that (i) miR-383-5p and miR-145-5p are downregulated in all BCC subtypes compared with control skin, (ii) miR-181c-5p is downregulated in superficial compared with invasive (nodular/infiltrative) BCC, and (iii) miR-22-5p and miR-708-5p are upregulated in infiltrative compared with superficial/nodular BCC and miR-30c-5p is downregulated in infiltrative compared with nodular BCC. Receiver operating characteristic analysis demonstrated excellent capacity of these miRs to discriminate between BCC and control skin (area under the curve, 0.94-0.98), whereas the capacity to discriminate between superficial and invasive subtypes was less robust (area under the curve, 0.7-0.8). Future prospective studies may determine the utility of these miRs as diagnostic biomarkers to guide biopsy and treatment of BCC.
摘要：
基底细胞癌（BCC）在组织学上分为确定治疗决定的亚型。microRNAs（miRs）是短的非编码RNA，可以作为诊断生物标志物。我们调查了特定miRs是否可以区分BCC亚型。我们对55个存档的BCC和9个对照皮肤标本的miRs进行了测序，然后通过qRT-PCR测定法在第二个BCC队列中验证了这些miRs（18个浅表，16个结节，15浸润性）和对照皮肤（n=12）。将单个miR的表达值标准化为miR-16-5p，这是对照皮肤和BCC样品中最少的变体。我们发现（i）与对照皮肤相比，所有BCC亚型中的miR-383-5p和miR-145-5p均下调，（ii）与侵袭性（结节/浸润性）BCC相比，miR-181c-5p在浅表中下调，和(iii)与浅表/结节性BCC相比,miR-22-5p和miR-708-5p在浸润性中上调,并且与结节性BCC相比,miR-30c-5p在浸润性中下调。接收器工作特性分析表明，这些miRs具有出色的区分BCC和对照皮肤的能力（曲线下面积，0.94-0.98），而区分浅层亚型和侵入性亚型的能力不太稳健(曲线下面积，0.7-0.8）。未来的前瞻性研究可能会确定这些miR作为诊断性生物标志物的实用性，以指导BCC的活检和治疗。
