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Abstract：
BACKGROUND: Gastric cancer (GC) has a high mortality rate, and robust diagnostic biomarkers are currently lacking. However, the clinical relevance of circular RNAs (circRNAs) as GC biomarkers remains largely unexplored.
OBJECTIVE: To evaluate the potential of novel circRNA circ_0004592 in the early screening and prognosis of GC.
METHODS: High-throughput sequencing of circRNAs was performed to screen for potential target molecules. Circ_0004592 expression was examined in GC tissues, cells, and plasma. Plasma samples were collected from healthy subjects\' patients, as well as from patients with benign lesions, precancerous lesions, and GC, whereafter the diagnostic accuracy of circ_0004592 was evaluated. The correlation between circ_0004592 levels in plasma and clinicopathological data of patients with GC was further analyzed.
RESULTS: Circ_0004592 was upregulated in both the tissue and plasma of patients with GC. Further, circ_0004592 expression was higher in patients with precancerous lesions than in healthy controls while being highest in patients with GC. In the same patient, the postoperative plasma level of circ_0004592 was lower than that in the preoperative period. Moreover, circ_0004592 level was significantly correlated with tumor differentiation, tumor depth, and lymph node metastasis. The area under the curve (AUC) of plasma circ_0004592 exhibited high sensitivity and specificity for differentiating patients with GC from healthy donors. Diagnosis based on circ_0004592, carcinoembryonic antigen, and cancer antigen 199 achieved a superior AUC and was highly sensitive.
CONCLUSIONS: Plasma circ_0004592 may represent a potential non-invasive auxiliary diagnostic biomarker for patients with GC.
OBJECTIVE: To evaluate the potential of novel circRNA circ_0004592 in the early screening and prognosis of GC.
METHODS: High-throughput sequencing of circRNAs was performed to screen for potential target molecules. Circ_0004592 expression was examined in GC tissues, cells, and plasma. Plasma samples were collected from healthy subjects\' patients, as well as from patients with benign lesions, precancerous lesions, and GC, whereafter the diagnostic accuracy of circ_0004592 was evaluated. The correlation between circ_0004592 levels in plasma and clinicopathological data of patients with GC was further analyzed.
RESULTS: Circ_0004592 was upregulated in both the tissue and plasma of patients with GC. Further, circ_0004592 expression was higher in patients with precancerous lesions than in healthy controls while being highest in patients with GC. In the same patient, the postoperative plasma level of circ_0004592 was lower than that in the preoperative period. Moreover, circ_0004592 level was significantly correlated with tumor differentiation, tumor depth, and lymph node metastasis. The area under the curve (AUC) of plasma circ_0004592 exhibited high sensitivity and specificity for differentiating patients with GC from healthy donors. Diagnosis based on circ_0004592, carcinoembryonic antigen, and cancer antigen 199 achieved a superior AUC and was highly sensitive.
CONCLUSIONS: Plasma circ_0004592 may represent a potential non-invasive auxiliary diagnostic biomarker for patients with GC.
摘要：
背景：胃癌（GC）的死亡率很高，和强大的诊断生物标志物目前缺乏。然而,环状RNA(circularRNAs,circRNAs)作为GC生物标志物的临床相关性在很大程度上仍未被研究.
目的：评估新型circRNAcirc_0004592在GC早期筛查和预后中的潜力。
方法：进行circRNAs的高通量测序以筛选潜在的靶分子。在GC组织中检查Circ_0004592表达，细胞,和等离子体。从健康受试者中收集血浆样本，以及良性病变患者，癌前病变,和GC，此后评估了circ_0004592的诊断准确性。进一步分析血浆中circ_0004592水平与GC患者临床病理资料的相关性。
结果：Circ_0004592在GC患者的组织和血浆中均上调。Further,癌前病变患者的circ_0004592表达高于健康对照组，而GC患者的表达最高。同一个病人,术后血浆circ_0004592水平低于术前水平。此外，circ_0004592水平与肿瘤分化显著相关，肿瘤深度,淋巴结转移。血浆circ_0004592的曲线下面积(AUC)表现出区分患有GC的患者与健康供体的高灵敏度和特异性。基于circ_0004592，癌胚抗原，和癌抗原199取得了优越的AUC和高度敏感。
结论：血浆circ_0004592可能是GC患者的一种潜在的非侵入性辅助诊断生物标志物。
目的：评估新型circRNAcirc_0004592在GC早期筛查和预后中的潜力。
方法：进行circRNAs的高通量测序以筛选潜在的靶分子。在GC组织中检查Circ_0004592表达，细胞,和等离子体。从健康受试者中收集血浆样本，以及良性病变患者，癌前病变,和GC，此后评估了circ_0004592的诊断准确性。进一步分析血浆中circ_0004592水平与GC患者临床病理资料的相关性。
结果：Circ_0004592在GC患者的组织和血浆中均上调。Further,癌前病变患者的circ_0004592表达高于健康对照组，而GC患者的表达最高。同一个病人,术后血浆circ_0004592水平低于术前水平。此外，circ_0004592水平与肿瘤分化显著相关，肿瘤深度,淋巴结转移。血浆circ_0004592的曲线下面积(AUC)表现出区分患有GC的患者与健康供体的高灵敏度和特异性。基于circ_0004592，癌胚抗原，和癌抗原199取得了优越的AUC和高度敏感。
结论：血浆circ_0004592可能是GC患者的一种潜在的非侵入性辅助诊断生物标志物。
