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Abstract：
UNASSIGNED: To explore the effect of Gouqi Nuzhen Liuhe Decoction (GNLHD) on the PI3K/mTOR Signaling Pathway for Premature Ovarian Insufficiency (POI) based on system pharmacology.
UNASSIGNED: First, the system pharmacology approach was used to predict the mechanism of GNLHD. Then, mice were randomly divided into model group, positive group, GNLHD high-dose group, GNLHD medium-dose group, and GNLHD low-dose group. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of ovarian tissue under light microscope. The expression levels of estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected by enzyme-linked immunosorbent assay. The expressions of PI3K, AKT1 and mTOR proteins in ovarian tissue were detected by immunohistochemistry.
UNASSIGNED: The results of system pharmacology showed that GNLHD may regulate biological processes and signaling pathways such as: reproductive structure development, reproductive system development, Oocyte meiosis and so on. Compared with the model group, the levels of E2 in the GNLHD group were increased, and the levels of FSH and LH were decreased (P < 0.05). Compared with the model group, the number of mature follicles in the GNLHD group was significantly increased, the number of atretic follicles was relatively decreased, and the expressions of PI3K, AKT1, and MTOR proteins in the GNLHD group were significantly increased (P < 0.05).
UNASSIGNED: GNLHD may improve the ovarian function of POI mice by affecting the expression of PI3K, AKT1 and mTOR proteins, promote the growth and development of follicles, increase the E2 level, reduce FSH and LH level, and maintain the stability of the ovarian internal environment.
UNASSIGNED: First, the system pharmacology approach was used to predict the mechanism of GNLHD. Then, mice were randomly divided into model group, positive group, GNLHD high-dose group, GNLHD medium-dose group, and GNLHD low-dose group. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of ovarian tissue under light microscope. The expression levels of estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected by enzyme-linked immunosorbent assay. The expressions of PI3K, AKT1 and mTOR proteins in ovarian tissue were detected by immunohistochemistry.
UNASSIGNED: The results of system pharmacology showed that GNLHD may regulate biological processes and signaling pathways such as: reproductive structure development, reproductive system development, Oocyte meiosis and so on. Compared with the model group, the levels of E2 in the GNLHD group were increased, and the levels of FSH and LH were decreased (P < 0.05). Compared with the model group, the number of mature follicles in the GNLHD group was significantly increased, the number of atretic follicles was relatively decreased, and the expressions of PI3K, AKT1, and MTOR proteins in the GNLHD group were significantly increased (P < 0.05).
UNASSIGNED: GNLHD may improve the ovarian function of POI mice by affecting the expression of PI3K, AKT1 and mTOR proteins, promote the growth and development of follicles, increase the E2 level, reduce FSH and LH level, and maintain the stability of the ovarian internal environment.
摘要：
■基于系统药理学探讨勾芪女贞六合汤(GNLHD)对早衰性卵巢功能不全(POI)PI3K/mTOR信号通路的影响。
■首先，系统药理学方法用于预测GNLHD的机制。然后，将小鼠随机分为模型组,阳性组，GNLHD高剂量组,GNLHD中剂量组,和GNLHD低剂量组。采用苏木精-伊红(HE)染色观察光镜下卵巢组织的病理变化。雌二醇（E2）的表达水平，采用酶联免疫吸附法检测卵泡刺激素(FSH)和黄体生成素(LH)。PI3K的表达式，免疫组化检测卵巢组织中AKT1和mTOR蛋白。
■系统药理学的结果表明，GNLHD可能调节生物过程和信号通路，例如：生殖结构发育，生殖系统发育,卵母细胞减数分裂等等。与模型组相比，GNLHD组E2水平升高,FSH和LH水平降低(P<0.05)。与模型组相比，GNLHD组成熟卵泡数量显著增加,闭锁卵泡的数量相对减少，和PI3K的表达式，GNLHD组AKT1、MTOR蛋白明显升高(P<0.05)。
■GNLHD可能通过影响PI3K的表达改善POI小鼠的卵巢功能，AKT1和mTOR蛋白，促进卵泡的生长发育，提高E2水平，降低FSH和LH水平，维持卵巢内环境的稳定。
■首先，系统药理学方法用于预测GNLHD的机制。然后，将小鼠随机分为模型组,阳性组，GNLHD高剂量组,GNLHD中剂量组,和GNLHD低剂量组。采用苏木精-伊红(HE)染色观察光镜下卵巢组织的病理变化。雌二醇（E2）的表达水平，采用酶联免疫吸附法检测卵泡刺激素(FSH)和黄体生成素(LH)。PI3K的表达式，免疫组化检测卵巢组织中AKT1和mTOR蛋白。
■系统药理学的结果表明，GNLHD可能调节生物过程和信号通路，例如：生殖结构发育，生殖系统发育,卵母细胞减数分裂等等。与模型组相比，GNLHD组E2水平升高,FSH和LH水平降低(P<0.05)。与模型组相比，GNLHD组成熟卵泡数量显著增加,闭锁卵泡的数量相对减少，和PI3K的表达式，GNLHD组AKT1、MTOR蛋白明显升高(P<0.05)。
■GNLHD可能通过影响PI3K的表达改善POI小鼠的卵巢功能，AKT1和mTOR蛋白，促进卵泡的生长发育，提高E2水平，降低FSH和LH水平，维持卵巢内环境的稳定。
