PDF(Pubmed)
Abstract：
UNASSIGNED: The skin plays a crucial role as a protective barrier against external factors, but disruptions to its integrity can lead to wound formation and hinder the natural healing process. Scar formation and delayed wound healing present significant challenges in skin injury treatment. While alternative approaches such as skin substitutes and tissue engineering exist, they are often limited in accessibility and cost. Exosomes have emerged as a potential solution for wound healing due to their regenerative properties.
UNASSIGNED: In this study, exosomes were isolated from human blood serum using a kit. The exosomes were characterized, and their effects on cell migration were assessed in vitro. Additionally, the wound healing capacity of exosomes was evaluated in vivo using a rat full-thickness wound model.
UNASSIGNED: Our in vitro findings revealed that exosomes significantly promoted cell migration. In vivo experiments demonstrated that the injection of exosomes at different areas of the wound accelerated the wound healing process, resulting in wound closure, collagen synthesis, vessel formation, and angiogenesis in the wound area. These results suggest that exosomes have a promising therapeutic potential for expediting wound healing and minimizing scar formation.
UNASSIGNED: The findings of this study highlight the potential of exosomes as a novel approach for enhancing wound healing. Exosomes showed positive effects on both cell migration and wound closure in in vitro and in vivo studies, suggesting their potential use as a regenerative therapy for skin injuries. Further research is needed to fully understand the mechanisms underlying the beneficial effects of exosomes on wound healing and to optimize their application in clinical settings.
UNASSIGNED: In this study, exosomes were isolated from human blood serum using a kit. The exosomes were characterized, and their effects on cell migration were assessed in vitro. Additionally, the wound healing capacity of exosomes was evaluated in vivo using a rat full-thickness wound model.
UNASSIGNED: Our in vitro findings revealed that exosomes significantly promoted cell migration. In vivo experiments demonstrated that the injection of exosomes at different areas of the wound accelerated the wound healing process, resulting in wound closure, collagen synthesis, vessel formation, and angiogenesis in the wound area. These results suggest that exosomes have a promising therapeutic potential for expediting wound healing and minimizing scar formation.
UNASSIGNED: The findings of this study highlight the potential of exosomes as a novel approach for enhancing wound healing. Exosomes showed positive effects on both cell migration and wound closure in in vitro and in vivo studies, suggesting their potential use as a regenerative therapy for skin injuries. Further research is needed to fully understand the mechanisms underlying the beneficial effects of exosomes on wound healing and to optimize their application in clinical settings.
摘要：
■皮肤作为抵御外部因素的保护屏障起着至关重要的作用，但是破坏其完整性会导致伤口形成并阻碍自然愈合过程。瘢痕形成和延迟的伤口愈合在皮肤损伤治疗中提出了重大挑战。虽然存在替代方法，如皮肤替代品和组织工程，它们通常在可访问性和成本方面受到限制。由于外泌体的再生特性，外泌体已成为伤口愈合的潜在解决方案。
■在这项研究中，使用试剂盒从人血清中分离外泌体。外泌体的特征是，并在体外评估了它们对细胞迁移的影响。此外,使用大鼠全层伤口模型在体内评估外泌体的伤口愈合能力。
■我们的体外研究结果表明，外泌体显著促进细胞迁移。体内实验表明，在伤口的不同区域注射外泌体加速了伤口的愈合过程，导致伤口闭合，胶原蛋白合成，血管形成,和伤口区域的血管生成。这些结果表明外泌体具有加速伤口愈合和最小化瘢痕形成的有希望的治疗潜力。
■这项研究的发现突出了外泌体作为增强伤口愈合的新方法的潜力。在体外和体内研究中，外泌体对细胞迁移和伤口闭合均显示出积极的作用，表明它们作为皮肤损伤的再生疗法的潜在用途。需要进一步的研究来充分了解外泌体对伤口愈合有益作用的潜在机制，并优化其在临床环境中的应用。
■在这项研究中，使用试剂盒从人血清中分离外泌体。外泌体的特征是，并在体外评估了它们对细胞迁移的影响。此外,使用大鼠全层伤口模型在体内评估外泌体的伤口愈合能力。
■我们的体外研究结果表明，外泌体显著促进细胞迁移。体内实验表明，在伤口的不同区域注射外泌体加速了伤口的愈合过程，导致伤口闭合，胶原蛋白合成，血管形成,和伤口区域的血管生成。这些结果表明外泌体具有加速伤口愈合和最小化瘢痕形成的有希望的治疗潜力。
■这项研究的发现突出了外泌体作为增强伤口愈合的新方法的潜力。在体外和体内研究中，外泌体对细胞迁移和伤口闭合均显示出积极的作用，表明它们作为皮肤损伤的再生疗法的潜在用途。需要进一步的研究来充分了解外泌体对伤口愈合有益作用的潜在机制，并优化其在临床环境中的应用。
