PDF(Pubmed)
Abstract：
Investigations in Wistar Albino Glaxo from Rijswijk (WAG/Rij) rats that are susceptible to genetic absence epilepsy have demonstrated that environmental modifications affect absence seizures. Previously, we showed that neonatal tactile stimulations produce disease-modifying effect on genetically determined absence epilepsy and associated depression in Wag/Rij rats. The study presented here examined the effect of TS during late ontogenesis (adolescence and young adulthood) on epilepsy and depression outcomes in this genetically epileptic rat strain. On postnatal day (PND) 38, male WAG/Rij rats randomly were assigned to either the tactile stimulation (TS), handled or control group (unhandled) with 8 animals in each group. Following a 7-day adaptation period to their new surroundings, the animals were submitted to tactile stimulation from PND 45 to PND 90, five days per week, for 5 min daily. The tactile-stimulated rat was removed from its cage, placed on the experimenter\'s lap, and had its neck and back gently stroked by the researcher. The handled rats were taken to another cage and left alone for 5 min daily from PND 45 to PND 90. The control rats were left undisturbed in their home cage, except for regular cage cleaning. After PND 90, all rats were left undisturbed until behavioral testing and EEG recording. When the animals were 7 months old, they were subjected to the sucrose consumption test (SCT) and the forced swimming test (FST). Electroencephalogram (EEG) recordings were made at 8 months of age in order to measure electroencephalographic seizure activity, thus, the spike-wave discharges (SWDs). Tactile-stimulated rats showed increased sucrose consumption and number of approaches to the sucrose solution in the SCT when compared with the handled and control rats. In the FST, rats in TS group showed lower immobility time and greater immobility latency, active swimming time and diving frequency than the handled and control rats. The duration and the number of seizures were not different amongst the groups. The data obtained suggest that TS in young rats is able to prevent depression in WAG/Rij rats.
摘要：
对来自Rijswijk（WAG/Rij）大鼠的WistarAlbinoGlaxo进行的研究表明，环境改变会影响癫痫发作。以前,我们发现,在Wag/Rij大鼠中,新生儿触觉刺激对遗传决定的失神癫痫和相关抑郁产生疾病改善作用.这里提出的研究检查了在这种遗传性癫痫大鼠品系中，TS在本体发育后期（青春期和成年期）对癫痫和抑郁结果的影响。在出生后第38天，将雄性WAG/Rij大鼠随机分配给触觉刺激（TS），处理组或对照组(未处理),每组8只动物。经过7天的适应新环境，动物接受从PND45到PND90的触觉刺激，每周五天，每天5分钟。从笼子里取出触觉刺激的大鼠，放在实验者的膝盖上，研究人员轻轻地抚摸着它的脖子和背部。将处理过的大鼠带到另一个笼子中,并每天从PND45到PND90单独放置5分钟。对照大鼠不受干扰地留在它们的笼子里，除了定期清洁笼子。PND90后，所有大鼠均保持不受干扰，直到行为测试和EEG记录。当动物7个月大的时候，进行蔗糖消耗试验(SCT)和强迫游泳试验(FST)。脑电图（EEG）记录在8个月大，以测量脑电图癫痫发作活动，因此，尖峰波放电(SWDs)。与处理和对照大鼠相比，触觉刺激的大鼠显示蔗糖消耗增加，SCT中蔗糖溶液的进路次数增加。在FST,TS组大鼠不动时间较短,不动潜伏期较大,主动游泳时间和潜水频率比处理和对照大鼠。两组之间的持续时间和癫痫发作次数没有差异。获得的数据表明，年轻大鼠的TS能够预防WAG/Rij大鼠的抑郁症。
