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Abstract：
Neurological pain (NP) is always accompanied by symptoms of depression, which seriously affects physical and mental health. In this study, we identified the common hub genes (Co-hub genes) and related immune cells of NP and major depressive disorder (MDD) to determine whether they have common pathological and molecular mechanisms. NP and MDD expression data was downloaded from the Gene Expression Omnibus (GEO) database. Common differentially expressed genes (Co-DEGs) for NP and MDD were extracted and the hub genes and hub nodes were mined. Co-DEGs, hub genes, and hub nodes were analyzed for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, the hub nodes, and genes were analyzed to obtain Co-hub genes. We plotted Receiver operating characteristic (ROC) curves to evaluate the diagnostic impact of the Co-hub genes on MDD and NP. We also identified the immune-infiltrating cell component by ssGSEA and analyzed the relationship. For the GO and KEGG enrichment analyses, 93 Co-DEGs were associated with biological processes (BP), such as fibrinolysis, cell composition (CC), such as tertiary granules, and pathways, such as complement, and coagulation cascades. A differential gene expression analysis revealed significant differences between the Co-hub genes ANGPT2, MMP9, PLAU, and TIMP2. There was some accuracy in the diagnosis of NP based on the expression of ANGPT2 and MMP9. Analysis of differences in the immune cell components indicated an abundance of activated dendritic cells, effector memory CD8+ T cells, memory B cells, and regulatory T cells in both groups, which were statistically significant. In summary, we identified 6 Co-hub genes and 4 immune cell types related to NP and MDD. Further studies are needed to determine the role of these genes and immune cells as potential diagnostic markers or therapeutic targets in NP and MDD.
摘要：
神经性疼痛（NP）总是伴有抑郁症状，严重影响身心健康。在这项研究中,我们鉴定了NP和重度抑郁障碍(MDD)的共同hub基因(Co-hub基因)和相关免疫细胞,以确定它们是否具有共同的病理和分子机制.从基因表达综合(GEO)数据库下载NP和MDD表达数据。提取了NP和MDD的常见差异表达基因（Co-DEGs），并挖掘了hub基因和hub节点。协同DEG,集线器基因，分析和枢纽节点的基因本体论（GO）和京都基因和基因组百科全书（KEGG）富集。最后,集线器节点，并对基因进行分析，获得Co-hub基因。我们绘制了受试者工作特征（ROC）曲线，以评估Co-hub基因对MDD和NP的诊断影响。我们还通过ssGSEA鉴定了免疫浸润细胞成分并分析了它们之间的关系。对于GO和KEGG富集分析，93个Co-DEGs与生物过程(BP)相关，如纤维蛋白溶解，细胞组成（CC），如三级颗粒，和路径，如补语，和凝结级联。差异基因表达分析显示Co-hub基因ANGPT2、MMP9、PLAU、和TIMP2。根据ANGPT2和MMP9的表达对NP的诊断有一定的准确性。对免疫细胞成分差异的分析表明，激活的树突状细胞丰富，效应记忆CD8+T细胞，记忆B细胞，两组的调节性T细胞，具有统计学意义。总之,我们确定了6个与NP和MDD相关的Co-hub基因和4种免疫细胞类型。需要进一步的研究来确定这些基因和免疫细胞作为NP和MDD中潜在的诊断标志物或治疗靶标的作用。
