PDF(Pubmed)
Abstract：
The harmful effects of blue light on the retina and health issues attributed to flickering light have been researched extensively. However, reports on the effects of flickering blue light at a frequency in the visible range on the retina are limited. This study aimed to non-invasively investigate the structural and functional changes in mice retinas following exposure to flickering blue light. BALB/c mice were subjected to non-flickering and flickering blue light, and changes in the retinal function and structure were assessed using electroretinography (ERG) and spectral-domain optical coherence tomography (SD-OCT), respectively. Retinal damage progression was monitored on days 3, 7, 14, and 42 following light exposure. Significant reductions in scotopic and photopic ERG responses were observed on day 3 (p<0.05). On day 7, the non-flickering and flickering groups demonstrated different functional changes: the flickering group showed further ERG response reduction, while the non-flickering group showed no reduction or slight improvement that was statistically insignificant (p>0.05). A similar trend lasted by day 14. On day 42, however, the difference between the non-flickering and flickering groups was significant, which was corroborated by the normalized amplitudes at 0, 0.5, and 1 log cd s/m2 (p<0.05). Quantitative and qualitative SD-OCT assays revealed more severe and progressive retinal damage in the flickering group throughout the study. Flickering blue light causes more persistent and severe retinal damage than non-flickering blue light and may be a risk factor for retinal degeneration even at frequencies as low as 20 Hz.
摘要：
蓝光对视网膜的有害影响和归因于闪烁光的健康问题已被广泛研究。然而,关于在可见范围内的频率闪烁的蓝光对视网膜的影响的报道是有限的。这项研究旨在非侵入性地研究暴露于闪烁的蓝光后小鼠视网膜的结构和功能变化。BALB/c小鼠受到非闪烁和闪烁的蓝光，使用视网膜电图（ERG）和谱域光学相干断层扫描（SD-OCT）评估视网膜功能和结构的变化，分别。在光暴露后第3、7、14和42天监测视网膜损伤进展。在第3天观察到暗视和明视ERG反应的显著降低(p<0.05)。第7天，非闪烁和闪烁组表现出不同的功能变化：闪烁组表现出进一步的ERG反应降低，而非闪烁组没有减少或轻微改善，统计学上无统计学意义（p>0.05）。类似的趋势持续到第14天。然而，在第42天，非闪烁组和闪烁组之间的差异是显著的，0、0.5和1logcds/m2的归一化幅度证实了这一点（p<0.05）。定量和定性SD-OCT测定显示在整个研究中闪烁组中更严重和进行性视网膜损伤。闪烁的蓝光比非闪烁的蓝光引起更持久和严重的视网膜损伤,并且即使在低至20Hz的频率下也可能是视网膜变性的风险因素。
