Abstract：
Spermatogonial stem cell (SSC) technologies that are currently under clinical development to reverse human infertility hold the potential to be adapted and applied for the conservation of endangered and vulnerable wildlife species. The biobanking of testis tissue containing SSCs from wildlife species, aligned with that occurring in pediatric human patients, could facilitate strategies to improve the genetic diversity and fitness of endangered populations. Approaches to utilize these SSCs could include spermatogonial transplantation or testis tissue grafting into a donor animal of the same or a closely related species, or in vitro spermatogenesis paired with assisted reproduction approaches. The primary roadblock to progress in this field is a lack of fundamental knowledge of SSC biology in non-model species. Herein, we review the current understanding of molecular mechanisms controlling SSC function in laboratory rodents and humans, and given our particular interest in the conservation of Australian marsupials, use a subset of these species as a case-study to demonstrate gaps-in-knowledge that are common to wildlife. Additionally, we review progress in the development and application of SSC technologies in fertility clinics and consider the translation potential of these techniques for species conservation pipelines.
摘要：
精原干细胞（SSC）技术目前正在临床开发中，以逆转人类不育症，具有适应和应用于保护濒危和脆弱野生动植物物种的潜力。含有来自野生动物物种的SSC的睾丸组织的生物带库，与儿科人类患者的情况一致，可以促进改善濒危种群遗传多样性和适应性的策略。利用这些SSC的方法可以包括精原移植或睾丸组织移植到相同或密切相关物种的供体动物中。或体外精子发生与辅助生殖方法配对。该领域取得进展的主要障碍是缺乏非模型物种中SSC生物学的基本知识。在这里，我们回顾了目前对实验室啮齿动物和人类控制SSC功能的分子机制的理解，考虑到我们对澳大利亚有袋动物的保护特别感兴趣，使用这些物种的子集作为案例研究，以证明野生生物常见的知识差距。此外,我们回顾了SSC技术在生育诊所中的开发和应用进展，并考虑了这些技术在物种保护管道中的翻译潜力。
