PDF(Pubmed)
Abstract：
Recapitulating the natural extracellular physical microenvironment has emerged as a promising method for tissue regeneration, as multiple physical interventions, including ultrasound, thermal and electrical therapy, have shown great potential. However, simultaneous coupling of multiple physical cues to highly bio-mimick natural characteristics for improved tissue regeneration still remains formidable. Coupling of intrinsic electrical and mechanical cues has been regarded as an effective way to modulate tissue repair. Nevertheless, precise and convenient manipulation on coupling of mechano-electrical signals within extracellular environment to facilitate tissue regeneration remains challengeable. Herein, a photothermal-sensitive piezoelectric membrane was designed for simultaneous integration of electrical and mechanical signals in response to NIR irradiation. The high-performance mechano-electrical coupling under NIR exposure synergistically triggered the promotion of osteogenic differentiation of stem cells and enhances bone defect regeneration by increasing cellular mechanical sensing, attachment, spreading and cytoskeleton remodeling. This study highlights the coupling of mechanical signals and electrical cues for modulation of osteogenesis, and sheds light on alternative bone tissue engineering therapies with multiple integrated physical cues for tissue repair.
摘要：
重述天然细胞外物理微环境已成为一种有前途的组织再生方法，作为多种物理干预措施，包括超声波,热和电治疗，显示出巨大的潜力。然而,同时将多种物理线索耦合到高度生物模仿的自然特征，以改善组织再生仍然是令人生畏的。内在电气和机械线索的耦合已被认为是调节组织修复的有效方法。然而，在细胞外环境内的机械电信号耦合以促进组织再生的精确和方便的操作仍然是具有挑战性的。在这里，设计了一种光热敏感压电膜，用于响应NIR辐射同时集成电信号和机械信号。NIR暴露下的高性能机电耦合协同触发促进干细胞成骨分化，并通过增加细胞力学感知来增强骨缺损再生，附件,扩散和细胞骨架重塑。这项研究强调了机械信号和电线索的耦合，以调节成骨，并阐明了具有多种整合的组织修复物理线索的替代骨组织工程疗法。
