PDF(Pubmed)
Abstract：
Cold stress poses a significant threat to the quality and productivity of lychee (Litchi chinensis Sonn.). While previous research has extensively explored the genomic and transcriptomic responses to cold stress in lychee, the translatome has not been thoroughly investigated. This study delves into the translatomic landscape of the \'Xiangjinfeng\' cultivar under both control and low-temperature conditions using RNA sequencing and ribosome profiling. We uncovered a significant divergence between the transcriptomic and translatomic responses to cold exposure. Additionally, bioinformatics analyses underscored the crucial role of codon occupancy in lychee\'s cold tolerance mechanisms. Our findings reveal that the modulation of translation via codon occupancy is a vital strategy to abiotic stress. Specifically, the study identifies ribosome stalling, particularly at the E site AAU codon, as a key element of the translation machinery in lychee\'s response to cold stress. This work enhances our understanding of the molecular dynamics of lychee\'s reaction to cold stress and emphasizes the essential role of translational regulation in the plant\'s environmental adaptability.
摘要：
冷胁迫对荔枝的品质和生产力构成重大威胁（荔枝).虽然以前的研究已经广泛探索了荔枝对冷应激的基因组和转录组反应，翻译酶还没有被彻底调查。本研究使用RNA测序和核糖体谱分析，在控制和低温条件下深入研究了“湘金丰”品种的翻译景观。我们发现了对冷暴露的转录组和翻译组反应之间的显着差异。此外,生物信息学分析强调了密码子占用在荔枝耐寒机制中的关键作用。我们的发现表明，通过密码子占据调节翻译是非生物胁迫的重要策略。具体来说,这项研究发现核糖体停滞，特别是在E位点的AAU密码子，作为荔枝应对寒冷压力的关键要素。这项工作增强了我们对荔枝对冷胁迫反应的分子动力学的理解，并强调了翻译调节在植物环境适应性中的重要作用。
