RESULTS: In total, 122 soybean BTB genes were identified, which were classified into four groups based on the phylogenetic analysis. Gene structures analysis indicated that the number of exon-intron in GmBTBs ranges from 0 to18. Cis-element analysis revealed that most GmBTB genes contained cis-elements related to an abiotic stress response. In addition, qRT-PCR analyses indicated that most GmBTBs are significantly up-regulated under salinity, drought, and nitrate stresses. They suggested their potential for targeted improvement of soybean response to multiple abiotic stresses and nitrate availability.
CONCLUSIONS: These results provide valuable information for identifying the members of the GmBTB gene family in soybean and could provide a functional characterization of GmBTB genes in further research.
结果:总计,已鉴定122个大豆BTB基因,根据系统发育分析将其分为四组。基因结构分析表明,GmBTBs中外显子-内含子的数量为0-18。顺式元素分析显示,大多数GmBTB基因包含与非生物胁迫反应相关的顺式元素。此外,qRT-PCR分析表明,大多数GmBTBs在盐度下显著上调,干旱,和硝酸盐应力。他们提出了有针对性地改善大豆对多种非生物胁迫和硝酸盐可用性的反应的潜力。
结论:这些结果为大豆中GmBTB基因家族成员的鉴定提供了有价值的信息,并且可以在进一步的研究中提供GmBTB基因的功能表征。