RESULTS: Expression level and genetic analyses revealed that the photoreceptor genes PHY, CRY, and PHOT have a negative impact on nematode infection. Interestingly, ELONGATED HYPOCOTYL5 (HY5), a downstream gene involved in the regulation of light signaling, is associated with photoreceptor-mediated negative regulation of root-knot nematode resistance. ChIP and yeast one-hybrid assays supported that HY5 participates in plant-to-root-knot nematode responses by directly binding to the SWEET negative regulatory factors involved in root-knot nematode resistance.
CONCLUSIONS: This study elucidates the important role of light signaling pathways in plant resistance to nematodes, providing a new perspective for RKN resistance research.
结果:表达水平和遗传分析表明,感光基因PHY,哭泣,和PHOT对线虫感染有负面影响。有趣的是,延长的下叶5(HY5),参与光信号调节的下游基因,与光感受器介导的根结线虫抗性负调控有关。ChIP和酵母单杂交测定法支持HY5通过直接结合参与根结线虫抗性的SWEET负调节因子来参与植物到根结线虫的反应。
结论:本研究阐明了光信号通路在植物对线虫的抗性中的重要作用,为RKN抗性研究提供了新的视角。