PDF(Pubmed)
Abstract：
BACKGROUND: Meloidogyne incognita is one of the most important plant-parasitic nematodes and causes tremendous losses to the agricultural economy. Light is an important living factor for plants and pathogenic organisms, and sufficient light promotes root-knot nematode infection, but the underlying mechanism is still unclear.
RESULTS: Expression level and genetic analyses revealed that the photoreceptor genes PHY, CRY, and PHOT have a negative impact on nematode infection. Interestingly, ELONGATED HYPOCOTYL5 (HY5), a downstream gene involved in the regulation of light signaling, is associated with photoreceptor-mediated negative regulation of root-knot nematode resistance. ChIP and yeast one-hybrid assays supported that HY5 participates in plant-to-root-knot nematode responses by directly binding to the SWEET negative regulatory factors involved in root-knot nematode resistance.
CONCLUSIONS: This study elucidates the important role of light signaling pathways in plant resistance to nematodes, providing a new perspective for RKN resistance research.
RESULTS: Expression level and genetic analyses revealed that the photoreceptor genes PHY, CRY, and PHOT have a negative impact on nematode infection. Interestingly, ELONGATED HYPOCOTYL5 (HY5), a downstream gene involved in the regulation of light signaling, is associated with photoreceptor-mediated negative regulation of root-knot nematode resistance. ChIP and yeast one-hybrid assays supported that HY5 participates in plant-to-root-knot nematode responses by directly binding to the SWEET negative regulatory factors involved in root-knot nematode resistance.
CONCLUSIONS: This study elucidates the important role of light signaling pathways in plant resistance to nematodes, providing a new perspective for RKN resistance research.
摘要：
背景：南方根结线虫是一种重要的植物寄生线虫，给农业经济造成了巨大的损失。光是植物和病原生物的重要生计因子，充足的光线促进根结线虫感染，但潜在的机制仍不清楚。
结果：表达水平和遗传分析表明，感光基因PHY，哭泣,和PHOT对线虫感染有负面影响。有趣的是,延长的下叶5（HY5），参与光信号调节的下游基因，与光感受器介导的根结线虫抗性负调控有关。ChIP和酵母单杂交测定法支持HY5通过直接结合参与根结线虫抗性的SWEET负调节因子来参与植物到根结线虫的反应。
结论：本研究阐明了光信号通路在植物对线虫的抗性中的重要作用，为RKN抗性研究提供了新的视角。
结果：表达水平和遗传分析表明，感光基因PHY，哭泣,和PHOT对线虫感染有负面影响。有趣的是,延长的下叶5（HY5），参与光信号调节的下游基因，与光感受器介导的根结线虫抗性负调控有关。ChIP和酵母单杂交测定法支持HY5通过直接结合参与根结线虫抗性的SWEET负调节因子来参与植物到根结线虫的反应。
结论：本研究阐明了光信号通路在植物对线虫的抗性中的重要作用，为RKN抗性研究提供了新的视角。
