PDF(Pubmed)
Abstract：
OBJECTIVE: Zinc Oxide nanoparticles (ZnO NPs) are used widely in nowadays personal care products, especially sunscreens, as a protector against UV irradiation. Yet, they have some reports of potential toxicity. Silica is widely used to cage ZnO NPs to reduce their potential toxicity. Vitamin C derivative, Magnesium Ascorpyl Phosphate (MAP), is a potent antioxidant that can efficiently protect human skin from harmful impacts of UV irradiation and oxidative stress. The combination of silica coated ZnO NPs and MAP nanovesicles could have potential synergistic protective effect against skin photodamage.
METHODS: Silica coated ZnO NPs and MAP nanovesicles (ethosomes and niosomes) were synthesized, formulated, and evaluated as topical gels. These gel formulations were evaluated in mice for their photoprotective effect against UV irradiation through histopathology and immuno-histochemistry study. Split-face clinical study was conducted to compare the effect of application of silica coated ZnO NPs either alone or combined with MAP nanovesicles. Their photoprotective action was evaluated, using Antera 3D® camera, for melanin level, roughness index and wrinkles depth.
RESULTS: Silica coated ZnO NPs when combined with MAP nanovesicles protected mice skin from UV irradiation and decreased the expression of the proinflammatory cytokines, NF-κB. Clinically, silica coated ZnO NPs, alone or combined with MAP nanovesicles, could have significant effect to decrease melanin level, roughness index and wrinkles depth with higher effect for the combination.
CONCLUSIONS: A composite of silica coated ZnO NPs and MAP nanovesicles could be a promising cosmetic formulation for skin protection against photodamage signs such as hyperpigmentation, roughness, and wrinkles.
METHODS: Silica coated ZnO NPs and MAP nanovesicles (ethosomes and niosomes) were synthesized, formulated, and evaluated as topical gels. These gel formulations were evaluated in mice for their photoprotective effect against UV irradiation through histopathology and immuno-histochemistry study. Split-face clinical study was conducted to compare the effect of application of silica coated ZnO NPs either alone or combined with MAP nanovesicles. Their photoprotective action was evaluated, using Antera 3D® camera, for melanin level, roughness index and wrinkles depth.
RESULTS: Silica coated ZnO NPs when combined with MAP nanovesicles protected mice skin from UV irradiation and decreased the expression of the proinflammatory cytokines, NF-κB. Clinically, silica coated ZnO NPs, alone or combined with MAP nanovesicles, could have significant effect to decrease melanin level, roughness index and wrinkles depth with higher effect for the combination.
CONCLUSIONS: A composite of silica coated ZnO NPs and MAP nanovesicles could be a promising cosmetic formulation for skin protection against photodamage signs such as hyperpigmentation, roughness, and wrinkles.
摘要：
目的：氧化锌纳米颗粒（ZnONPs）广泛应用于当今的个人护理产品中，尤其是防晒霜,作为紫外线照射的保护者。然而，他们有一些潜在毒性的报道。二氧化硅广泛用于笼罩着ZnONPs以降低其潜在的毒性。维生素C衍生物，抗坏血酸磷酸镁（MAP），是一种有效的抗氧化剂，可以有效地保护人类皮肤免受紫外线照射和氧化应激的有害影响。二氧化硅涂覆的ZnONP和MAP纳米囊泡的组合可以具有针对皮肤光损伤的潜在协同保护作用。
方法：合成了二氧化硅包覆的ZnONPs和MAP纳米囊泡（乙醇体和囊泡），制定，并评估为局部凝胶。通过组织病理学和免疫组织化学研究，在小鼠中评估了这些凝胶制剂对紫外线照射的光保护作用。进行了分面临床研究，以比较单独或与MAP纳米囊泡组合使用二氧化硅包覆的ZnONP的效果。评估了它们的光保护作用，使用Antera3D®相机，黑色素水平，粗糙度指数和皱纹深度。
结果：二氧化硅包覆的ZnONPs与MAP纳米囊泡结合时，可保护小鼠皮肤免受紫外线照射，并降低促炎细胞因子的表达，NF-κB.临床上,二氧化硅包覆ZnONPs，单独或与MAP纳米囊泡结合，可能对降低黑色素水平有显著影响，粗糙度指数和皱纹深度具有较高的组合效果。
结论：二氧化硅涂覆的ZnONP和MAP纳米囊泡的复合材料可能是一种有前途的化妆品配方，用于保护皮肤免受光损伤迹象，如色素沉着过度，粗糙度,和皱纹。
方法：合成了二氧化硅包覆的ZnONPs和MAP纳米囊泡（乙醇体和囊泡），制定，并评估为局部凝胶。通过组织病理学和免疫组织化学研究，在小鼠中评估了这些凝胶制剂对紫外线照射的光保护作用。进行了分面临床研究，以比较单独或与MAP纳米囊泡组合使用二氧化硅包覆的ZnONP的效果。评估了它们的光保护作用，使用Antera3D®相机，黑色素水平，粗糙度指数和皱纹深度。
结果：二氧化硅包覆的ZnONPs与MAP纳米囊泡结合时，可保护小鼠皮肤免受紫外线照射，并降低促炎细胞因子的表达，NF-κB.临床上,二氧化硅包覆ZnONPs，单独或与MAP纳米囊泡结合，可能对降低黑色素水平有显著影响，粗糙度指数和皱纹深度具有较高的组合效果。
结论：二氧化硅涂覆的ZnONP和MAP纳米囊泡的复合材料可能是一种有前途的化妆品配方，用于保护皮肤免受光损伤迹象，如色素沉着过度，粗糙度,和皱纹。
