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Abstract：
To study the effects of attenuated Salmonella typhimurium L forms on the in vivo tumorigenicity and apoptosis of murine epithelial ovarian cancer cells, as well as the related mechanisms. Attenuated Salmonella typhimurium VNP20009 was induced into bacterial L forms by using antibiotic ceftriaxone. CCK-8 cell proliferation assay showed that attenuated S. typhimurium L forms can inhibit the proliferation of murine ovarian epithelial cancer ID8 cells. Attenuated ST L forms can induce apoptosis and inhibit invasion ability of epithelial ovarian cancer cells in vitro. TUNEL assay showed that attenuated ST L forms can induce apoptosis of ID8 cells in murine ovarian tumors. Meanwhile, attenuated ST L forms inhibit tumor growth in murine ovarian tumors. The tumorigenicity-related proteins of xenograft tumors detected by immunohistochemistry and fluorescence quantitative RT-PCR assays showed that attenuated ST L forms can reduce the expression of proteins that promote tumor growth and metastasis, such as Lgals9 and MMP9. This study confirmed that attenuated ST L forms can suppress tumor growth and promote apoptosis in murine ovarian tumors. Attenuated ST L forms may serve as a novel biological agent for bacterial-mediated tumor therapy in epithelial ovarian cancer.
摘要：
研究减毒鼠伤寒沙门氏菌L型对小鼠上皮性卵巢癌细胞体内致瘤性和凋亡的影响。以及相关机制。使用抗生素头孢曲松将减毒的鼠伤寒沙门氏菌VNP20009诱导为细菌L型。CCK-8细胞增殖实验表明，减毒鼠伤寒沙门氏菌L型可抑制小鼠卵巢上皮性癌ID8细胞的增殖。减毒STL型在体外可诱导上皮性卵巢癌细胞凋亡并抑制其侵袭能力。TUNEL分析表明，减毒的STL形式可以诱导小鼠卵巢肿瘤中ID8细胞的凋亡。同时,减毒的STL形式抑制小鼠卵巢肿瘤的肿瘤生长。通过免疫组织化学和荧光定量RT-PCR检测异种移植肿瘤的致瘤性相关蛋白，结果表明，减毒STL型可降低促进肿瘤生长和转移的蛋白表达，例如Lgals9和MMP9。这项研究证实，减毒的STL形式可以抑制小鼠卵巢肿瘤的肿瘤生长并促进细胞凋亡。减毒的STL形式可作为上皮性卵巢癌中细菌介导的肿瘤治疗的新型生物制剂。
