PDF(Pubmed)
Abstract：
While second near-infrared (NIR-II) fluorescence imaging is a promising tool for real-time surveillance of surgical operations, the previously reported organic NIR-II luminescent materials for in vivo imaging are predominantly activated by expensive lasers or X-ray with high power and poor illumination homogeneity, which significantly limits their clinical applications. Here we report a white-light activatable NIR-II organic imaging agent by taking advantages of the strong intramolecular/intermolecular D-A interactions of conjugated Y6CT molecules in nanoparticles (Y6CT-NPs), with the brightness of as high as 13315.1, which is over two times that of the brightest laser-activated NIR-II organic contrast agents reported thus far. Upon white-light activation, Y6CT-NPs can achieve not only in vivo imaging of hepatic ischemia reperfusion, but also real-time monitoring of kidney transplantation surgery. During the surgery, identification of the renal vasculature, post-reconstruction assessment of renal allograft vascular integrity, and blood supply analysis of the ureter can be vividly depicted by using Y6CT-NPs with high signal-to-noise ratios upon clinical laparoscopic LED white-light activation. Our work provides efficient molecular design guidelines towards white-light activatable imaging agent and highlights an opportunity for precision imaging theranostics.
摘要：
虽然第二近红外（NIR-II）荧光成像是实时监测外科手术的有前途的工具，先前报道的用于体内成像的有机NIR-II发光材料主要由昂贵的激光或X射线激活，这极大地限制了它们的临床应用。在这里，我们通过利用纳米粒子（Y6CT-NP）中共轭Y6CT分子的强分子内/分子间D-A相互作用来报告白光可激活的NIR-II有机显像剂，亮度高达13315.1，是迄今为止报道的最亮的激光激活的NIR-II有机造影剂的两倍以上。白光激活后，Y6CT-NPs不仅可以实现肝脏缺血再灌注的活体成像,还可以实时监测肾移植手术。在手术过程中,肾脉管系统的鉴定，移植肾血管完整性重建后评估,通过在临床腹腔镜LED白光激活后使用具有高信噪比的Y6CT-NP，可以生动地描述输尿管的血液供应分析。我们的工作为白光可激活的显像剂提供了有效的分子设计指南，并为精密成像疗法提供了机会。
