PDF(Pubmed)
Abstract：
The establishment of symbiotic interactions between leguminous plants and rhizobia requires complex cellular programming activated by Rhizobium Nod factors (NFs) as well as type III effector (T3E)-mediated symbiotic signaling. However, the mechanisms by which different signals jointly affect symbiosis are still unclear. Here we describe the mechanisms mediating the cross-talk between the broad host range rhizobia Sinorhizobium fredii HH103 T3E Nodulation Outer Protein L (NopL) effector and NF signaling in soybean. NopL physically interacts with the Glycine max Remorin 1a (GmREM1a) and the NFs receptor NFR5 (GmNFR5) and promotes GmNFR5 recruitment by GmREM1a. Furthermore, NopL and NF influence the expression of GmRINRK1, a receptor-like kinase (LRR-RLK) ortholog of the Lotus RINRK1, that mediates NF signaling. Taken together, our work indicates that S. fredii NopL can interact with the NF signaling cascade components to promote the symbiotic interaction in soybean.
摘要：
豆科植物和根瘤菌之间共生相互作用的建立需要由根瘤菌Nod因子（NFs）以及III型效应子（T3E）介导的共生信号激活的复杂细胞编程。然而,不同信号共同影响共生的机制尚不清楚。在这里，我们描述了介导广泛寄主根瘤菌中根瘤菌HH103T3E结瘤外部蛋白L（NopL）效应子和大豆中NF信号传导之间的串扰的机制。NopL与甘氨酸maxRemorin1a（GmREM1a）和NFs受体NFR5（GmNFR5）物理相互作用，并通过GmREM1a促进GmNFR5募集。此外，NopL和NF影响GmRINRK1的表达，GmRINRK1是LotusRINRK1的受体样激酶（LRR-RLK）直系同源物，可介导NF信号传导。一起来看,我们的工作表明，S.frediiNopL可以与NF信号级联组分相互作用，以促进大豆中的共生相互作用。
