PDF(Pubmed)
Abstract：
Embryonic germ cells develop rapidly to establish the foundation for future developmental trajectories, and in this process, they make critical lineage choices including the configuration of their unique identity and a decision on sex. Here, we use single-cell genomics patterns for the entire embryonic germline in Drosophila melanogaster along with the somatic gonadal precursors after embryonic gonad coalescence to investigate molecular mechanisms involved in the setting up and regulation of the germline program. Profiling of the early germline chromatin landscape revealed sex- and stage-specific features. In the male germline immediately after zygotic activation, the chromatin structure underwent a brief remodeling phase during which nucleosome density was lower and deconcentrated from promoter regions. These findings echoed enrichment analysis results of our genomics data in which top candidates were factors with the ability to mediate large-scale chromatin reorganization. Together, they point to the importance of chromatin regulation in the early germline and raise the possibility of a conserved epigenetic reprogramming-like process required for proper initiation of germline development.
摘要：
胚胎生殖细胞迅速发展，为未来的发育轨迹奠定了基础，在这个过程中,他们做出关键的血统选择，包括他们独特身份的配置和性别的决定。这里,我们使用黑腹果蝇整个胚胎种系的单细胞基因组学模式以及胚胎性腺合并后的体细胞性腺前体，以研究建立和调节种系程序的分子机制。早期种系染色质景观的分析揭示了性别和阶段特定的特征。在合子激活后立即在雄性种系中，染色质结构经历了短暂的重塑阶段，在此期间，核小体密度较低，并且从启动子区域分散。这些发现与我们基因组学数据的富集分析结果相呼应，其中顶级候选物是具有介导大规模染色质重组能力的因子。一起,他们指出染色质调节在早期种系中的重要性,并提高了适当启动种系发育所需的保守的表观遗传重编程样过程的可能性.
