Abstract：
Continuous glucose monitors are crucial for diabetes management, but invasive sampling, signal drift and frequent calibrations restrict their widespread usage. Microneedle sensors are emerging as a minimally-invasive platform for real-time monitoring of clinical parameters in interstitial fluid. Herein, a painless and flexible microneedle sensing patch is constructed by a mechanically-strong microneedle base and a thin layer of fluorescent hydrogel sensor for on-site, accurate, and continuous glucose monitoring. The Förster resonance energy transfer (FRET)-based hydrogel sensors are fabricated by facile photopolymerizations of acryloylated FRET pairs and glucose-specific phenylboronic acid. The optimized hydrogel sensor enables quantification of glucose with reversibility, high selectivity, and signal stability against photobleaching. Poly (ethylene glycol diacrylate)-co-polyacrylamide hydrogel is utilized as the microneedle base, facilitating effective skin piercing and biofluid extraction. The integrated microneedle sensor patch displays a sensitivity of 0.029 mM-1 in the (patho)physiological range, a low detection limit of 0.193 mM, and a response time of 7.7 min in human serum. Hypoglycemia, euglycemia and hyperglycemia are continuously monitored over 6 h simulated meal and rest activities in a porcine skin model. This microneedle sensor with high transdermal analytical performance offers a powerful tool for continuous diabetes monitoring at point-of-care settings.
摘要：
连续血糖监测仪对糖尿病管理至关重要，但是侵入性采样，信号漂移和频繁的校准限制了它们的广泛使用。微针传感器正在成为一种微创平台，用于实时监测间质液中的临床参数。在这里，一个无痛和灵活的微针传感贴片是由一个机械坚固的微针底座和一层薄的荧光水凝胶传感器构建的，准确,和连续血糖监测。基于Förster共振能量转移（FRET）的水凝胶传感器是通过丙烯酰化FRET对和葡萄糖特异性苯基硼酸的容易的光聚合来制造的。优化的水凝胶传感器可实现葡萄糖的可逆性定量，高选择性,和抗光漂白的信号稳定性。聚（乙二醇二丙烯酸酯）-共聚聚丙烯酰胺水凝胶用作微针基底，促进有效的皮肤穿刺和生物流体提取。集成的微针传感器贴片在（patho）生理范围内显示0.029mM-1的灵敏度，0.193mM的低检测限，在人血清中的响应时间为7.7分钟。低血糖,在猪皮肤模型中,持续监测正常血糖和高血糖超过6小时的模拟进餐和休息活动。这种具有高透皮分析性能的微针传感器提供了一个强大的工具，用于在护理点环境中连续监测糖尿病。
