Abstract：
The current study was constructed to fabricate polyamide based nanofibrous scaffolds (NS) and to define the most promising one for the generation of cardiomyocytes from adipose tissue derived mesenchymal stem cells (ADMSCs). This purpose was extended to assess the potentiality of the generated cardiomyocytes in relieving myocardial infarction (MI) in rats. Production and characterization of NSs were carried out. ADMSCs were cultured on NS and induced to differentiate into cardiomyocytes by specific growth factors. Molecular analysis for myocyte-specific enhancer factor 2 C (MEF2C) and alpha sarcomeric actin (α-SCA) expression was done to confirm the differentiation of ADMSCs into cardiomyocytes for further transplantation into MI induced rats. Implantation of cells in MI afflicted rats boosted heart rate, ST height and PR interval and lessened P duration, RR, QTc and QRS intervals. Also, this type of medication minified serum lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) enzymes activity as well as serum and cardiac troponin T (Tn-T) levels and upraised serum and cardiac α-SCA and cardiac connexin 43 (CX 43) levels. Microscopic feature of cardiac tissue sections of rats in the treated groups revealed great renovation in the cardiac microarchitecture. Conclusively, this attempt gains insight into a realistic strategy for recovery of MI through systemic employment of in vitro generated cardiomyocytes.
摘要：
本研究旨在制造基于聚酰胺的纳米纤维支架（NS），并定义了从脂肪组织来源的间充质干细胞（ADMSCs）产生心肌细胞的最有前途的支架。此目的扩展到评估产生的心肌细胞在缓解大鼠心肌梗塞（MI）中的潜力。进行了NS的生产和表征。将ADMSCs在NS上培养，并通过特异性生长因子诱导分化为心肌细胞。对心肌细胞特异性增强因子2C（MEF2C）和α肌节肌动蛋白（α-SCA）表达进行了分子分析，以确认ADMSCs分化为心肌细胞，以进一步移植到MI诱导的大鼠中。在MI患病的大鼠中植入细胞可提高心率，ST段高度和PR间隔，缩短P持续时间，RR,QTc和QRS间隔。此外，这种类型的药物降低了血清乳酸脱氢酶（LDH）和肌酸激酶-MB（CK-MB）酶的活性以及血清和心肌肌钙蛋白T（Tn-T）水平，并提高了血清和心脏α-SCA和心脏连接蛋白43（CX43）水平。治疗组大鼠心脏组织切片的微观特征揭示了心脏微观结构的巨大更新。最后，这项尝试深入了解了通过全身使用体外产生的心肌细胞来恢复MI的现实策略.
