Abstract：
OBJECTIVE: To investigate the protective effect of intravesical glucosamine in treating overactive bladder (OAB).
METHODS: Ninety-two female Sprague-Dawley (SD) rats were divided into 4 groups i.e. protamine sulfate (PS), N-acetylcysteine (NAC), and glucosamine-treated PS (GPS), and normal saline control (NC) were used. We induced hyperactivity in rats via intravesical infusion of PS and potassium chloride (KCl), whereas the NC group underwent a sustained intravesical saline infusion for 1 h. N-acetylcysteine (NAC), a potential antioxidant as well as anti-inflammatory agent was employed as positive control. Cystometrography (CMG) was then conducted to determine urodynamic parameters, i.e., leak point pressure (LPP, n = 48) and inter-contractile interval, the duration between two voids (ICI, n = 32).
RESULTS: LPP was significantly elevated in the GPS group (mean ± SD: 110.9 ± 6.2 mmHg) compared to the NC (81.0 ± 32.5 mmHg), PS (40.3 ± 10.9 mmHg), and NAC group (70.3 ± 19.4 mmHg). The cystometrogram data also reveals a prolonged ICI in the GPS group (241.3 ± 40.2 s) compared to the NC group (216.0 ± 41.7 s), PS group (128.8 ± 23.6 s), and NAC group (193.8 ± 28.3 s).
CONCLUSIONS: This preliminary study implies the ameliorative impact of GPS treatment on OAB in terms of improved urodynamic parameters, including LPP and ICI.
METHODS: Ninety-two female Sprague-Dawley (SD) rats were divided into 4 groups i.e. protamine sulfate (PS), N-acetylcysteine (NAC), and glucosamine-treated PS (GPS), and normal saline control (NC) were used. We induced hyperactivity in rats via intravesical infusion of PS and potassium chloride (KCl), whereas the NC group underwent a sustained intravesical saline infusion for 1 h. N-acetylcysteine (NAC), a potential antioxidant as well as anti-inflammatory agent was employed as positive control. Cystometrography (CMG) was then conducted to determine urodynamic parameters, i.e., leak point pressure (LPP, n = 48) and inter-contractile interval, the duration between two voids (ICI, n = 32).
RESULTS: LPP was significantly elevated in the GPS group (mean ± SD: 110.9 ± 6.2 mmHg) compared to the NC (81.0 ± 32.5 mmHg), PS (40.3 ± 10.9 mmHg), and NAC group (70.3 ± 19.4 mmHg). The cystometrogram data also reveals a prolonged ICI in the GPS group (241.3 ± 40.2 s) compared to the NC group (216.0 ± 41.7 s), PS group (128.8 ± 23.6 s), and NAC group (193.8 ± 28.3 s).
CONCLUSIONS: This preliminary study implies the ameliorative impact of GPS treatment on OAB in terms of improved urodynamic parameters, including LPP and ICI.
摘要：
目的：探讨氨基葡萄糖对膀胱过度活动症(OAB)的保护作用。
方法：将92只雌性SD大鼠分为4组，即硫酸鱼精蛋白（PS），N-乙酰半胱氨酸(NAC),和葡萄糖胺处理的PS（GPS），使用生理盐水对照(NC)。我们通过膀胱灌注PS和氯化钾（KCl）诱导大鼠多动症，而NC组接受持续膀胱内生理盐水输注1小时。N-乙酰半胱氨酸（NAC），潜在的抗氧化剂和抗炎剂被用作阳性对照.然后进行膀胱造影（CMG）以确定尿动力学参数，即，泄漏点压力(LPP，n=48)和收缩间隔，两个空隙之间的持续时间(ICI,n=32)。
结果：与NC组（81.0±32.5mmHg）相比，GPS组的LPP显着升高（平均值±SD：110.9±6.2mmHg），PS（40.3±10.9mmHg），NAC组(70.3±19.4mmHg)。细胞图数据还显示，与NC组（216.0±41.7s）相比，GPS组的ICI延长（241.3±40.2s），PS组（128.8±23.6s），NAC组(193.8±28.3s)。
结论：这项初步研究暗示了GPS治疗在改善尿动力学参数方面对OAB的改善影响，包括LPP和ICI。
方法：将92只雌性SD大鼠分为4组，即硫酸鱼精蛋白（PS），N-乙酰半胱氨酸(NAC),和葡萄糖胺处理的PS（GPS），使用生理盐水对照(NC)。我们通过膀胱灌注PS和氯化钾（KCl）诱导大鼠多动症，而NC组接受持续膀胱内生理盐水输注1小时。N-乙酰半胱氨酸（NAC），潜在的抗氧化剂和抗炎剂被用作阳性对照.然后进行膀胱造影（CMG）以确定尿动力学参数，即，泄漏点压力(LPP，n=48)和收缩间隔，两个空隙之间的持续时间(ICI,n=32)。
结果：与NC组（81.0±32.5mmHg）相比，GPS组的LPP显着升高（平均值±SD：110.9±6.2mmHg），PS（40.3±10.9mmHg），NAC组(70.3±19.4mmHg)。细胞图数据还显示，与NC组（216.0±41.7s）相比，GPS组的ICI延长（241.3±40.2s），PS组（128.8±23.6s），NAC组(193.8±28.3s)。
结论：这项初步研究暗示了GPS治疗在改善尿动力学参数方面对OAB的改善影响，包括LPP和ICI。
