Abstract：
The animal product most used as a stimulatory additive for cell cultivation is still fetal bovine serum (FBS). Besides the ethical concerns regarding serum collection, the main problems of FBS are batch-to-batch variability and the resulting risk of lower reproducibility, the differences between species, the presence of undefined/unknown components, and the risk of contamination. In contrast, pig blood, which is a by-product of slaughter, is a sufficiently available and sustainable resource with a high degree of standardization in terms of donor age, weight, and genetics. The variations in preparations from pig slaughter blood seem to be comparatively low, and consequently, batch effects might be much smaller, suggesting that the reproducibility of the research data obtained may be increased. Our pilot study aimed to investigate, as a proof of concept, whether adult human and porcine stem cells of different tissue origins proliferate and differentiate adequately when FBS is completely or partially replaced by porcine serum (PS). We could show that the human and porcine stem cells were vital and proliferated under partial and full PS supplementation. Furthermore, using PS, the two cell types studied showed tissue-specific differentiation (i.e., lipid vacuoles as a sign of adipogenic or myotubes as a sign of myogenic differentiation). In conclusion, the pig slaughter blood-derived serum has promising potential to be a replacement for FBS in adult stem cell cultures. Therefore, it could serve as a basis for the development of new cell culture supplements.
摘要：
最多用作细胞培养的刺激性添加剂的动物产品仍然是胎牛血清(FBS)。除了关于血清采集的伦理问题，FBS的主要问题是批次间的可变性以及由此产生的重复性较低的风险，物种之间的差异，存在未定义/未知的组件，和污染的风险。相比之下,猪血,这是屠宰的副产品，是一种充分可用和可持续的资源，在供体年龄方面具有高度标准化，体重,和遗传学。猪屠宰血制剂的变化似乎相对较低，因此，批处理效果可能要小得多，这表明所获得的研究数据的可重复性可能会增加。我们的试点研究旨在调查，作为概念的证明，当FBS被猪血清（PS）完全或部分替代时，不同组织来源的成人和猪干细胞是否充分增殖和分化。我们可以证明，在部分和全部PS补充下，人和猪干细胞是至关重要的，并且可以增殖。此外，使用PS，研究的两种细胞类型显示出组织特异性分化(即，脂质液泡作为脂肪形成的标志或肌管作为肌源性分化的标志）。总之,猪屠宰血液来源的血清有望替代成体干细胞培养中的FBS。因此,它可以作为开发新的细胞培养补充剂的基础。
