Abstract：
The present investigation has evaluated the use of effluents from a secondary municipal wastewater treatment plant for biomass production and potential of the biomass for biodiesel production. Cultivations of Chlorella vulgaris using wastewater, wastewater with supplementation, and WC medium were carried out. Effect of wastewater collected in different months on biomass productivity (BP) and lipid composition was studied. Methods based on NMR and GC-MS techniques were applied for determining the composition of the lipids and their fatty acid profile including poly unsaturated fatty acids (PUFAs). Lipids extracted are comprised of both neutral (tri acyl glycerides, TAG; free fatty acids, FFA) and polar (glyco glycero/phospho) lipids. The TAG content of the extracted lipids was determined in the range of 22.5-41.3% w/w. The NMR and GC-MS compositional results of microalgal lipids of biomasses cultivated in wastewater without nutrient supplementation, collected in different months, showed potential for biodiesel production. The fatty acid profiles of neutral and polar lipids, which are mainly comprised of saturated and unsaturated long alkyl chain (C16-C22) fatty acids, are potential sources for the biodiesel and food industry. The concentration of nitrates (45-78 mg L-1) in wastewater without supplementation, collected in different months, was found to be optimum to enable cultivation of biomasses with reasonably good BP of 21.5-28.1 mg L-1 day-1. Similar results have been obtained in the present work as well as reported in the literature in the case of WC medium (nitrate, 69 mg L-1) with BP of 25.5-28.2 mg L-1 day-1, thus highlighted the significance of the presented work.
摘要：
本调查评估了二级市政污水处理厂的废水用于生物质生产以及生物质用于生物柴油生产的潜力。利用废水培养小球藻，废水补充，和WC培养基进行。研究了不同月份收集的废水对生物量生产率（BP）和脂质组成的影响。基于NMR和GC-MS技术的方法用于确定脂质的组成及其脂肪酸谱，包括多不饱和脂肪酸（PUFA）。提取的脂质由中性（三酰基甘油酯，标签；游离脂肪酸，FFA）和极性（甘油/磷酸）脂质。提取的脂质的TAG含量在22.5-41.3%w/w的范围内测定。在没有营养补充的情况下，在废水中培养的生物质的微藻脂质的NMR和GC-MS组成结果，在不同月份收集，显示了生物柴油生产潜力。中性和极性脂质的脂肪酸谱，主要由饱和和不饱和长烷基链（C16-C22）脂肪酸组成，是生物柴油和食品工业的潜在来源。废水中硝酸盐的浓度（45-78mgL-1）没有补充，在不同月份收集，被发现最适合培养具有21.5-28.1mgL-1day-1的良好BP的生物质。在目前的工作中获得了类似的结果，并且在WC介质（硝酸盐，69mgL-1），BP为25.5-28.2mgL-1day-1，因此强调了本工作的重要性。
