Abstract：
Lymphatics are involved in the resolution of inflammation and wound healing, but their role in the oral wound healing process after tooth extraction has never been investigated. We therefore sought to evaluate the healing process following the extraction of maxillary molars in two transgenic mouse models: K14-VEGFR3-Ig mice, which lack initial mucosal lymphatic vessels, and K14-VEGFC mice, which have hyperplastic mucosal lymphatics. Maxillary molars were extracted from both transgenic mouse types and their corresponding wild-type (WT) controls. Mucosal and alveolar bone healing were evaluated. A delayed epithelialization and bone regeneration were observed in K14-VEGFR3-Ig mice compared with their WT littermates. The hampered wound closure was accompanied by decreased levels of epidermal growth factor (EGF) and persistent inflammation, characterized by infiltrates of immune cells and elevated levels of pro-inflammatory markers in the wounds. Hyperplastic mucosal lymphatics did not enhance the healing process after tooth extraction in K14-VEGFC mice. The findings indicate that initial mucosal lymphatics play a major role in the initial phase of the oral wound healing process.
摘要：
淋巴管参与炎症和伤口愈合的解决，但是它们在拔牙后口腔伤口愈合过程中的作用从未被研究过。因此，我们试图评估在两个转基因小鼠模型中提取上颌磨牙后的愈合过程：K14-VEGFR3-Ig小鼠，缺乏初始粘膜淋巴管，和K14-VEGFC小鼠，有增生性粘膜淋巴管。从两种转基因小鼠类型及其相应的野生型（WT）对照中提取上颌磨牙。评估粘膜和牙槽骨的愈合情况。与WT同窝相比，在K14-VEGFR3-Ig小鼠中观察到延迟的上皮形成和骨再生。受阻碍的伤口闭合伴随着表皮生长因子（EGF）水平的降低和持续的炎症，特征在于伤口中的免疫细胞浸润和促炎标志物水平升高。在K14-VEGFC小鼠中，增生性粘膜淋巴管不能增强拔牙后的愈合过程。研究结果表明，初始粘膜淋巴管在口腔伤口愈合过程的初始阶段中起主要作用。
