Abstract：
In the pursuit of advancing neural tissue regeneration, biomaterial scaffolds have emerged as promising candidates, offering potential solutions for nerve disruptions. Among these scaffolds, multichannel hydrogels, characterized by meticulously designed micrometer-scale channels, stand out as instrumental tools for guiding axonal growth and facilitating cellular interactions. This study explores the innovative application of human amniotic membranes modified with methacryloyl domains (AMMA) in neural stem cell (NSC) culture. AMMA hydrogels, possessing a tailored softness resembling the physiological environment, are prepared in the format of multichannel scaffolds to simulate native-like microarchitecture of nerve tracts. Preliminary experiments on AMMA hydrogel films showcase their potential for neural applications, demonstrating robust adhesion, proliferation, and differentiation of NSCs without the need for additional coatings. Transitioning into the 3D realm, the multichannel architecture fosters intricate neuronal networks guiding neurite extension longitudinally. Furthermore, the presence of synaptic vesicles within the cellular arrays suggests the establishment of functional synaptic connections, underscoring the physiological relevance of the developed neuronal networks. This work contributes to the ongoing efforts to find ethical, clinically translatable, and functionally relevant approaches for regenerative neuroscience.
摘要：
为了促进神经组织再生，生物材料支架已经成为有希望的候选者，为神经中断提供潜在的解决方案。在这些脚手架中，多通道水凝胶，其特点是精心设计的微米尺度通道，作为引导轴突生长和促进细胞相互作用的工具。本研究探讨了用甲基丙烯酰结构域（AMMA）修饰的人羊膜在神经干细胞（NSC）培养中的创新应用。AMMA水凝胶，具有类似于生理环境的定制柔软度，以多通道支架的形式制备，以模拟神经束的天然样微结构。AMMA水凝胶膜的初步实验展示了它们在神经应用中的潜力，表现出强大的附着力，扩散,和NSC的分化，而不需要额外的涂层。过渡到3D领域，多通道结构促进了复杂的神经元网络，引导神经突纵向延伸。此外，细胞阵列中突触小泡的存在表明功能性突触连接的建立，强调了发达神经元网络的生理相关性。这项工作有助于不断努力寻找道德，临床可翻译，以及再生神经科学的功能相关方法。
