PDF(Pubmed)
Abstract：
The effects of Lactobacillus plantarum in microencapsulation (LPM) on intestinal development in layer chicks were investigated in this study, as well as the colonization of L. plantarum in the gut. A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups (8 replicates each treatment), and the diets of these birds were supplemented with nothing (control), L. plantarum (0.02 g/kg feed; 109 CFU/kg feed), LPM (1.0 g/kg feed; 109 CFU/kg feed) and wall material of LPM (WM; 0.98 g/kg feed), respectively. Compared to control, LPM improved growth performance and intestinal development of layer chicks, evidenced by significantly increased body weight, average daily gain, average daily feed intake, villus height, villus height/crypt depth, as well as weight and length of the duodenum, jejunum and ileum (P < 0.05). These results could be attributed to the increased colonization of L. plantarum in the gut, which was verified by significant increases in lactic acid content, viable counts in chyme and mucosa (P < 0.05), as well as a visible rise in number of strains labeled with fluorescein isothiocyanate. Meanwhile, the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated L. plantarum supplementation (P < 0.05), accompanied by the significant up-regulation of colonization related genes (P < 0.05), encoding solute carrier family, monocarboxylate transporter, activin A receptor, succinate receptor and secretogranin II. To sum up, microencapsulated L. plantarum supplementation promoted intestinal development, which could be attributed to the enhancement of L. plantarum colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.
摘要：
本研究研究了微囊化植物乳杆菌(LPM)对蛋鸡肠道发育的影响,以及植物乳杆菌在肠道中的定植。将480只健康0d龄的Hy-Line棕色蛋鸡随机分为4组(每组8个重复)，这些鸟的饮食没有任何补充（对照），植物乳杆菌（0.02g/kg饲料；109CFU/kg饲料），LPM（1.0g/kg饲料；109CFU/kg饲料）和LPM的壁材（WM；0.98g/kg饲料），分别。与控制相比，LPM改善了蛋鸡的生长性能和肠道发育，体重显著增加，平均每日收益，平均日采食量，绒毛高度,绒毛高度/隐窝深度,以及十二指肠的重量和长度，空肠和回肠(P<0.05)。这些结果可归因于植物乳杆菌在肠道中的定植增加,乳酸含量的显著增加证实了这一点，食糜和粘膜活菌计数（P<0.05），以及用异硫氰酸荧光素标记的菌株数量明显增加。同时,补充微囊化植物乳杆菌和双歧杆菌的相对丰度显着增加（P<0.05），伴随着定植相关基因的显著上调(P<0.05),编码溶质载体家族，单羧酸盐转运蛋白，激活素A受体,琥珀酸受体和分泌腺素II。总而言之,微囊化植物乳杆菌补充剂促进肠道发育，这可能归因于通过双歧杆菌的互助以及与定植相关的跨膜蛋白的相互作用来增强植物乳杆菌在肠道中的定植。
