PDF(Pubmed)
Abstract：
UNASSIGNED: Diode laser is one of the most captivating technologies in dental practice. In periodontics, when used at appropriate settings, it possesses the best properties for selective surgical and nonsurgical procedures such as subgingival calculus removal without a thermal change of the root surface, and also provides tissue surface sterilization. However, lasers always produce a certain amount of thermal damage to the soft tissues. Therefore, this study aimed to comparatively evaluate the thermal impact of 980-nm diode laser incisions, when used with irrigation versus nonirrigation systems.
UNASSIGNED: The study was conducted on 24 gingival tissue samples obtained from the goat\'s mandible and maxilla. The samples were divided into two groups of 12 samples each, and laser incisions were given in 4 power settings, using 980-nm diode laser in super short pulse mode, with and without saline irrigation. Martius Scarlet Blue staining technique was used to prepare the histological slides. Then, slides were examined under a research microscope (Olympus CX 21), and the pictures of the slides were taken by mounting the camera onto the microscope mobile mount. The microscopic images hence obtained were analyzed for the depth and width of the incisions, area of carbonization, necrosis, and reversible damage, using the Digimizer image analysis software.
UNASSIGNED: The results of this study demonstrated that the mean incision depth was higher (592.49 ± 180.97, P < 0.05), with less carbonization (25.52 ± 29.21, P = 0.00) and less necrosis (311.63 ± 156.441, P < 0.05) in the laser incisions with irrigation, as compared to the laser incisions given without irrigation system.
UNASSIGNED: Within the limitations of this study, it can be concluded that using an irrigation system causes less collateral damage while maintaining the incising efficiency of the diode laser. Further studies with a higher sample size, controlled irrigation systems, and incision techniques are needed to evaluate the efficiency of diode lasers for the clinical explanation of the results.
UNASSIGNED: The study was conducted on 24 gingival tissue samples obtained from the goat\'s mandible and maxilla. The samples were divided into two groups of 12 samples each, and laser incisions were given in 4 power settings, using 980-nm diode laser in super short pulse mode, with and without saline irrigation. Martius Scarlet Blue staining technique was used to prepare the histological slides. Then, slides were examined under a research microscope (Olympus CX 21), and the pictures of the slides were taken by mounting the camera onto the microscope mobile mount. The microscopic images hence obtained were analyzed for the depth and width of the incisions, area of carbonization, necrosis, and reversible damage, using the Digimizer image analysis software.
UNASSIGNED: The results of this study demonstrated that the mean incision depth was higher (592.49 ± 180.97, P < 0.05), with less carbonization (25.52 ± 29.21, P = 0.00) and less necrosis (311.63 ± 156.441, P < 0.05) in the laser incisions with irrigation, as compared to the laser incisions given without irrigation system.
UNASSIGNED: Within the limitations of this study, it can be concluded that using an irrigation system causes less collateral damage while maintaining the incising efficiency of the diode laser. Further studies with a higher sample size, controlled irrigation systems, and incision techniques are needed to evaluate the efficiency of diode lasers for the clinical explanation of the results.
摘要：
■二极管激光是牙科实践中最迷人的技术之一。在牙周病中，在适当的设置下使用时，它具有选择性手术和非手术程序的最佳特性，例如在不发生根面热变化的情况下去除龈下结石，并且还提供组织表面灭菌。然而,激光总是对软组织产生一定量的热损伤。因此,这项研究旨在比较评估980纳米二极管激光切口的热影响，当与灌溉和非灌溉系统一起使用时。
■该研究是对从山羊下颌骨和上颌骨获得的24个牙龈组织样本进行的。样本分为两组，每组12个样本，激光切口在4个功率设置下给出，在超短脉冲模式下使用980nm二极管激光器，有和没有盐水灌溉。采用Martius猩蓝染色技术制备组织学载玻片。然后，在研究显微镜下检查载玻片（OlympusCX21），通过将相机安装到显微镜移动支架上来拍摄载玻片的照片。分析由此获得的显微图像的切口的深度和宽度。碳化面积，坏死,和可逆的损伤，使用Digimizer图像分析软件。
■这项研究的结果表明，平均切口深度更高（592.49±180.97，P<0.05），冲洗激光切口碳化少（25.52±29.21，P=0.00），坏死少（311.63±156.441，P<0.05），与没有灌溉系统的激光切口相比。
■在本研究的局限性内，可以得出的结论是，使用灌溉系统引起较少的附带损害，同时保持二极管激光器的切割效率。更高样本量的进一步研究，控制灌溉系统，和切口技术需要评估二极管激光器的效率，以对结果进行临床解释。
■该研究是对从山羊下颌骨和上颌骨获得的24个牙龈组织样本进行的。样本分为两组，每组12个样本，激光切口在4个功率设置下给出，在超短脉冲模式下使用980nm二极管激光器，有和没有盐水灌溉。采用Martius猩蓝染色技术制备组织学载玻片。然后，在研究显微镜下检查载玻片（OlympusCX21），通过将相机安装到显微镜移动支架上来拍摄载玻片的照片。分析由此获得的显微图像的切口的深度和宽度。碳化面积，坏死,和可逆的损伤，使用Digimizer图像分析软件。
■这项研究的结果表明，平均切口深度更高（592.49±180.97，P<0.05），冲洗激光切口碳化少（25.52±29.21，P=0.00），坏死少（311.63±156.441，P<0.05），与没有灌溉系统的激光切口相比。
■在本研究的局限性内，可以得出的结论是，使用灌溉系统引起较少的附带损害，同时保持二极管激光器的切割效率。更高样本量的进一步研究，控制灌溉系统，和切口技术需要评估二极管激光器的效率，以对结果进行临床解释。
