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Abstract：
UNASSIGNED: Colorectal cancer (CRC) remains the leading cause of cancer death worldwide. Less than half of the patients are diagnosed when the cancer is locally advanced. Several studies have shown that intelectin-1 (ITLN1) can serve as a key prognostic and therapeutic target for CRC. The purpose of this study was to investigate the clinical value of ITLN1 in CRC and to analyse its potential as a predictive biomarker for CRC.
UNASSIGNED: Colon adenocarcinoma (COAD) is the main type of CRC. COAD project in The Cancer Genome Atlas (TCGA) database served as the training cohort, and GSE39582 series in the Gene Expression Omnibus (GEO) database served as the external independent validation cohort. First, the difference in the expression level of ITLN1 between COAD tissue and normal tissue was analysed, and the results were verified via immunohistochemistry. The relationship between ITLN1 expression and the prognosis of COAD patients was evaluated via the heatmap and the Kaplan-Meier (KM) curve. The ITLN1 coexpressed gene set obtained by Pearson correlation analysis was used. The prognostic signatures that were significantly correlated with survival status were screened by Cox and least absolute shrinkage and selection operator (LASSO) regression analyses. Finally, a nomogram related to ITLN1 was constructed based on the risk score of the prognostic signature and routine clinicopathological variables.
UNASSIGNED: ITLN1 is significantly underexpressed in tumour tissues and can be used as a valuable tool to distinguish COAD. The high-expression group of ITLN1 was verified to have a greater survival rate. ITLN1 is significantly associated with a good prognosis in COAD patients. Six candidate genes (ITLN1 and MORC2, SH2D7, LGALS4, ATOH1, and NAT2) were selected for use in the Cox-LASSO regression analysis to calculate the risk score. Finally, a nomogram was constructed with a comprehensive risk score and clinicopathologic factors to successfully predict and verify the 1-year, 3-year, and 5-year survival probability.
UNASSIGNED: Our study established ITLN1 as an effective tool for CRC screening, diagnosis, and prognostic assessment, provided a basis for further study of the molecular function of ITLN1, and provided new insights for the mechanistic exploration and treatment of CRC.
UNASSIGNED: Colon adenocarcinoma (COAD) is the main type of CRC. COAD project in The Cancer Genome Atlas (TCGA) database served as the training cohort, and GSE39582 series in the Gene Expression Omnibus (GEO) database served as the external independent validation cohort. First, the difference in the expression level of ITLN1 between COAD tissue and normal tissue was analysed, and the results were verified via immunohistochemistry. The relationship between ITLN1 expression and the prognosis of COAD patients was evaluated via the heatmap and the Kaplan-Meier (KM) curve. The ITLN1 coexpressed gene set obtained by Pearson correlation analysis was used. The prognostic signatures that were significantly correlated with survival status were screened by Cox and least absolute shrinkage and selection operator (LASSO) regression analyses. Finally, a nomogram related to ITLN1 was constructed based on the risk score of the prognostic signature and routine clinicopathological variables.
UNASSIGNED: ITLN1 is significantly underexpressed in tumour tissues and can be used as a valuable tool to distinguish COAD. The high-expression group of ITLN1 was verified to have a greater survival rate. ITLN1 is significantly associated with a good prognosis in COAD patients. Six candidate genes (ITLN1 and MORC2, SH2D7, LGALS4, ATOH1, and NAT2) were selected for use in the Cox-LASSO regression analysis to calculate the risk score. Finally, a nomogram was constructed with a comprehensive risk score and clinicopathologic factors to successfully predict and verify the 1-year, 3-year, and 5-year survival probability.
UNASSIGNED: Our study established ITLN1 as an effective tool for CRC screening, diagnosis, and prognostic assessment, provided a basis for further study of the molecular function of ITLN1, and provided new insights for the mechanistic exploration and treatment of CRC.
摘要：
结直肠癌（CRC）仍然是全球癌症死亡的主要原因。不到一半的患者在癌症局部晚期时被诊断出来。多项研究表明，intelectin-1（ITLN1）可以作为CRC的关键预后和治疗靶标。这项研究的目的是研究ITLN1在CRC中的临床价值，并分析其作为CRC预测生物标志物的潜力。
■结肠腺癌（COAD）是CRC的主要类型。癌症基因组图谱（TCGA）数据库中的COAD项目作为培训队列，基因表达综合(GEO)数据库中的GSE39582系列作为外部独立验证队列。首先,分析了COAD组织与正常组织之间ITLN1表达水平的差异，结果经免疫组化证实。通过热图和Kaplan-Meier(KM)曲线评价ITLN1表达与COAD患者预后的关系。使用通过Pearson相关性分析获得的ITLN1共表达基因集。通过Cox和最小绝对收缩和选择算子（LASSO）回归分析筛选与生存状态显着相关的预后特征。最后,根据预后特征的风险评分和常规临床病理变量,构建了与ITLN1相关的列线图.
■ITLN1在肿瘤组织中表达显着不足，可用作区分COAD的有价值的工具。证实ITLN1高表达组有较高的存活率。ITLN1与COAD患者的良好预后显著相关。选择六个候选基因（ITLN1和MORC2，SH2D7，LGALS4，ATOH1和NAT2）用于Cox-LASSO回归分析以计算风险评分。最后,用综合风险评分和临床病理因素构建列线图，以成功预测和验证1年，3年,和5年生存概率。
■我们的研究确立了ITLN1作为CRC筛查的有效工具，诊断,和预后评估，为进一步研究ITLN1的分子功能奠定了基础,为CRC的机制探索和治疗提供了新的见解。
■结肠腺癌（COAD）是CRC的主要类型。癌症基因组图谱（TCGA）数据库中的COAD项目作为培训队列，基因表达综合(GEO)数据库中的GSE39582系列作为外部独立验证队列。首先,分析了COAD组织与正常组织之间ITLN1表达水平的差异，结果经免疫组化证实。通过热图和Kaplan-Meier(KM)曲线评价ITLN1表达与COAD患者预后的关系。使用通过Pearson相关性分析获得的ITLN1共表达基因集。通过Cox和最小绝对收缩和选择算子（LASSO）回归分析筛选与生存状态显着相关的预后特征。最后,根据预后特征的风险评分和常规临床病理变量,构建了与ITLN1相关的列线图.
■ITLN1在肿瘤组织中表达显着不足，可用作区分COAD的有价值的工具。证实ITLN1高表达组有较高的存活率。ITLN1与COAD患者的良好预后显著相关。选择六个候选基因（ITLN1和MORC2，SH2D7，LGALS4，ATOH1和NAT2）用于Cox-LASSO回归分析以计算风险评分。最后,用综合风险评分和临床病理因素构建列线图，以成功预测和验证1年，3年,和5年生存概率。
■我们的研究确立了ITLN1作为CRC筛查的有效工具，诊断,和预后评估，为进一步研究ITLN1的分子功能奠定了基础,为CRC的机制探索和治疗提供了新的见解。
