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Abstract：
UNASSIGNED: Centromere protein U (CENPU) is key for mitosis in the carcinogenesis of cancers. However, the roles of CENPU have not been inspected in nasopharyngeal carcinoma (NPC). Thus, we aimed to explore the functions and mechanisms of CENPU in NPC.
UNASSIGNED: Expression of CENPU was evaluated by real-time quantitative polymerase chain reaction, western blotting and immunohistochemistry. The biological functions of CENPU were evaluated in vitro and in vivo. Gene chip analysis, ingenuity pathway analysis, and coimmunoprecipitation experiments were used to explore the mechanisms of CENPU.
UNASSIGNED: CENPU was highly expressed in NPC. High expression of CENPU was associated with advanced tumor, node and metastasis (TNM) stage and poor overall survival. Cox regression analysis demonstrated that CENPU expression was an independent prognostic factor in NPC. Knockdown of CENPU inhibited proliferation and migration in vitro and in vivo. Knockdown of CENPU upregulated dual specificity phosphatase 6 (DUSP6) expression. The expression of CNEPU was inversely correlated with the expression of DUSP6 in NPC tissues. Mechanistic studies confirmed that CENPU increased the activation of the ERK1/2 and p38 signaling pathways by suppressing the expression of DUSP6.
UNASSIGNED: CENPU acts as an oncogene in NPC by interacting with DUSP6, and may represent a promising prognostic biomarker for patients with NPC.
UNASSIGNED: Expression of CENPU was evaluated by real-time quantitative polymerase chain reaction, western blotting and immunohistochemistry. The biological functions of CENPU were evaluated in vitro and in vivo. Gene chip analysis, ingenuity pathway analysis, and coimmunoprecipitation experiments were used to explore the mechanisms of CENPU.
UNASSIGNED: CENPU was highly expressed in NPC. High expression of CENPU was associated with advanced tumor, node and metastasis (TNM) stage and poor overall survival. Cox regression analysis demonstrated that CENPU expression was an independent prognostic factor in NPC. Knockdown of CENPU inhibited proliferation and migration in vitro and in vivo. Knockdown of CENPU upregulated dual specificity phosphatase 6 (DUSP6) expression. The expression of CNEPU was inversely correlated with the expression of DUSP6 in NPC tissues. Mechanistic studies confirmed that CENPU increased the activation of the ERK1/2 and p38 signaling pathways by suppressing the expression of DUSP6.
UNASSIGNED: CENPU acts as an oncogene in NPC by interacting with DUSP6, and may represent a promising prognostic biomarker for patients with NPC.
摘要：
■着丝粒蛋白U（CENPU）是癌症发生中有丝分裂的关键。然而,尚未检查CENPU在鼻咽癌（NPC）中的作用。因此，我们旨在探讨CENPU在鼻咽癌中的作用和机制。
■通过实时定量聚合酶链反应评估CENPU的表达，蛋白质印迹和免疫组织化学。在体外和体内评估了CENPU的生物学功能。基因芯片分析,独创性途径分析，并通过免疫共沉淀实验探讨了CENPU的作用机制。
■CENPU在NPC中高表达。CENPU高表达与肿瘤进展有关，淋巴结和转移（TNM）分期和总体生存率低。Cox回归分析显示，CENPU表达是鼻咽癌的独立预后因素。敲除CENPU在体外和体内抑制增殖和迁移。敲除CENPU上调双特异性磷酸酶6(DUSP6)表达。CNEPU的表达与DUSP6的表达呈负相关。机制研究证实，CENPU通过抑制DUSP6的表达增加了ERK1/2和p38信号通路的激活。
■CENPU通过与DUSP6相互作用而在NPC中充当癌基因，并且可能代表NPC患者的有希望的预后生物标志物。
■通过实时定量聚合酶链反应评估CENPU的表达，蛋白质印迹和免疫组织化学。在体外和体内评估了CENPU的生物学功能。基因芯片分析,独创性途径分析，并通过免疫共沉淀实验探讨了CENPU的作用机制。
■CENPU在NPC中高表达。CENPU高表达与肿瘤进展有关，淋巴结和转移（TNM）分期和总体生存率低。Cox回归分析显示，CENPU表达是鼻咽癌的独立预后因素。敲除CENPU在体外和体内抑制增殖和迁移。敲除CENPU上调双特异性磷酸酶6(DUSP6)表达。CNEPU的表达与DUSP6的表达呈负相关。机制研究证实，CENPU通过抑制DUSP6的表达增加了ERK1/2和p38信号通路的激活。
■CENPU通过与DUSP6相互作用而在NPC中充当癌基因，并且可能代表NPC患者的有希望的预后生物标志物。
