PDF(Pubmed)
Abstract：
Introduction: The production of bone-like structural scaffolds through bone tissue engineering technology is a promising method for bone regeneration to repair bone defects. Deer antler, an easily harvested and abundantly sourced initial bone tissue structure, resembles the composition and structure of human cancellous bone and can serve as a new material for allogeneic bone transplantation. Methods: This study involved the preparation and characterization of antler powder/chitosan/β-glycerophosphate sodium/polyvinyl alcohol (AP/CS/β-GP/PVA) porous hydrogel scaffolds to verify their material properties and osteogenic mechanisms. The microstructure, hydrophilicity, and mechanical properties of the scaffolds were studied using Scanning Electron Microscopy (SEM), contact angle measurement, and a universal material testing machine. The interactions between the various components were investigated using Fourier-Transform Infrared Spectroscopy (FTIR). Biocompatibility, osteogenic properties, and expression of osteogenesis-related proteins of the scaffolds were evaluated through Cell Counting Kit-8 (CCK-8) assays, alkaline phosphatase staining, Alizarin Red staining, live/dead cell staining, and Western blot analysis. Results: The results showed that as the content of deer antler powder increased, both the hydrophilicity and mechanical properties of the scaffold materials improved, while the porosity slightly decreased with an increase in deer antler powder content. Cell culture experiments demonstrated that scaffolds with a higher proportion of deer antler powder were beneficial for the proliferation and differentiation of mouse pre-osteoblast (MC3T3-E1) cells, with the scaffolds containing 10% and 8% deer antler powder showing the best effects. The upregulation of RUNX2, OCN, OSX, and OPN protein expression may promote differentiation. Discussion: Therefore, the AP/CS/β-GP/PVA hydrogel scaffolds have the potential to become a promising biomaterial for bone tissue engineering.
摘要：
简介：通过骨组织工程技术生产骨样结构支架是一种有前途的骨再生修复骨缺损的方法。鹿茸,易于收获且来源丰富的初始骨组织结构，类似于人松质骨的组成和结构，可以作为同种异体骨移植的新材料。方法：本研究涉及鹿茸粉/壳聚糖/β-甘油磷酸钠/聚乙烯醇（AP/CS/β-GP/PVA）多孔水凝胶支架的制备和表征，以验证其材料性能和成骨机制。微观结构,亲水性,使用扫描电子显微镜（SEM）研究了支架的力学性能，接触角测量,和万能材料试验机。使用傅立叶变换红外光谱（FTIR）研究了各种成分之间的相互作用。生物相容性,成骨特性，并通过细胞计数试剂盒-8（CCK-8）测定评估支架的成骨相关蛋白的表达，碱性磷酸酶染色，茜素红染色,活/死细胞染色，和蛋白质印迹分析。结果:随着鹿茸粉含量的增加,支架材料的亲水性和力学性能均得到改善,随着鹿茸粉含量的增加，孔隙率略有降低。细胞培养实验表明，鹿茸粉比例较高的支架有利于小鼠前成骨细胞（MC3T3-E1）细胞的增殖和分化。支架中含有10%和8%鹿茸粉的效果最好。RUNX2，OCN的上调，OSX,OPN蛋白表达可能促进分化。讨论：因此，AP/CS/β-GP/PVA水凝胶支架具有成为骨组织工程生物材料的潜力。
