PDF(Pubmed)
Abstract：
Mitochondrial ferritin (FtMt) is a novel ferritin that sequesters iron and plays a protective role against oxidative stress. FtMt shares a high homology with H-ferritin but is expressed only in the brain, heart, and testis. In the midbrain, FtMt expression is observed in the substantia nigra. FtMt plays a neuroprotective role in the pathology of neurodegenerative diseases such as Parkinson\'s disease, where excessive iron induces oxidative stress, causing cell death. Herein, we investigated FtMt immunoreactivity in the brains of patients with subarachnoid hemorrhage (SAH). Double immunofluorescence labeling of tyrosine hydroxylase (TH) and FtMt showed high colocalization in the substantia nigra pars compacta (SNc) in control and SAH cases. However, in SAH cases, FtMt immunoreactivity was observed in some TH-negative neurons. Double immunofluorescence labeling of glial cell markers and FtMt showed no apparent colocalization. The number and ratio of FtMt-positive but TH-negative neurons significantly differed between the control and SAH groups. Prussian blue staining in SAH cases showed positive iron staining over a wide surface range and the substantia nigra. Thus, FtMt may be related to iron dynamics in the substantia nigra following subarachnoid hemorrhage.
摘要：
线粒体铁蛋白（FtMt）是一种新型的铁蛋白，可螯合铁并对氧化应激起保护作用。FtMt与H-铁蛋白具有高度同源性，但仅在大脑中表达，心,和睾丸。在中脑，在黑质中观察到FtMt表达。FtMt在帕金森病等神经退行性疾病的病理学中起神经保护作用。过量的铁诱导氧化应激，导致细胞死亡。在这里，我们研究了蛛网膜下腔出血（SAH）患者大脑中的FtMt免疫反应性。在对照组和SAH病例中，酪氨酸羟化酶（TH）和FtMt的双重免疫荧光标记在黑质致密部（SNc）中显示出高度的共定位。然而,在SAH病例中，在一些TH阴性神经元中观察到FtMt免疫反应性。神经胶质细胞标记和FtMt的双重免疫荧光标记显示没有明显的共定位。对照组和SAH组之间FtMt阳性但TH阴性神经元的数量和比例显着不同。SAH病例中的普鲁士蓝染色显示在较宽的表面范围和黑质上呈阳性铁染色。因此，FtMt可能与蛛网膜下腔出血后黑质中的铁动力学有关。
