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Abstract：
Quisqualis fructus (QF) is a traditional Chinese medicine (TCM) that it has a long history in the therapeutic field of killing parasites, eliminating accumulation, and stopping diarrhea. However, the therapeutic material basis of QF is remaining ambiguous nowadays. The geographical origin differences of QF are also usually ignored in the process of medication. In this study, the alcohol-aqueous soluble constituents in QF from different origins were systematically characterized and accurately measured by ultra-high performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and high-performance liquid chromatography (HPLC) respectively. Chemometric analysis was performed for origin differentiation and screening of potential quality marker (Q-marker). Finally, A total of 106 constituents were tentatively characterized in positive and negative ion modes, including 29 fatty acids, 26 organic acids, 11 amino acids and derivatives, 10 glycosides, 9 alkaloids and derivatives, and 21 other constituents. QF from different origins were effectively distinguished and 16 constituents were selected as the potential Q-markers subsequently. Four representative components (trigonelline, adenosine, ellagic acid, and 3,3\'-di-O-methylellagic acid) in QF samples were simultaneously determined. HPLC fingerprint analysis indicated that the similarity between 16 batches of QF was in the range of 0.870-0.999. The above results provide some insights for the research on the pharmacodynamic constituents, quality control, and geographical discrimination of QF.
摘要：
Quisqualisfructus(QF)是一种传统的中药(TCM)，它在杀寄生虫的治疗领域有着悠久的历史，消除积累，停止腹泻。然而,目前QF的治疗物质基础仍然模棱两可。在用药过程中，QF的地理起源差异也通常被忽略。在这项研究中,分别采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)和高效液相色谱(HPLC)对不同来源QF中的醇-水溶性成分进行了系统表征和准确测定。进行化学计量学分析以用于来源分化和潜在质量标记(Q-标记)的筛选。最后,总共106个成分在正离子和负离子模式下进行了初步表征，包括29种脂肪酸，26有机酸，11氨基酸及其衍生物，10个糖苷，9生物碱及其衍生物，和其他21个组成部分。有效区分了来自不同来源的QF，随后选择了16种成分作为潜在的Q标记。四种有代表性的成分(Trigonelline，腺苷,鞣花酸,同时测定QF样品中的3,3'-二-O-甲基鞣花酸）。HPLC指纹图谱分析表明，16批QF的相似度在0.870-0.999之间。以上结果为药效学成分的研究提供了一些启示,质量控制,和QF的地理歧视。
