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Abstract：
Catheter ablation has been demonstrated to reduce atrial fibrillation (AF) recurrence. The mechanisms of AF recurrence after catheter ablation are unknown, and the present study aimed to identify serum proteins associated with AF recurrence. The present prospective study comprised a cohort of patients with AF, which was divided into two groups after one-year follow-up: group 1 included patients with compensated AF after catheter ablation and group 2 included patients with AF recurrence after catheter ablation. Initial microarray profiling of the serum proteins was performed in small subgroups M1 and M2 recruited from groups 1 and 2, respectively, by an antibody microarray to evaluate potentially relevant proteins. The data of initial proteomic profiling identified candidate proteins in groups 1 and 2, and their levels were then measured by ELISA. The data of profiling suggested an overall increase in the levels of RAD51 and p63 proteins in the M2 subgroup versus that in the M1 subgroup, indicating potential relevance of these two proteins to AF recurrence. The results of ELISA of the levels of RAD51 and p63 in the groups 1 and 2 demonstrated an increase in the levels of RAD51 (11.11 ± 4.36 vs 8.45 ± 4.85 ng/mL; P = 0.009) and p63 (165.73 ± 113.75 vs 100.05 ± 37.56 units of normalized optical density; P = 0.0007) in the group 2 (with AF recurrence or substrate AF) compared with that in the group 1 (compensated AF). Thus, RAD51 and p63 were associated with AF recurrence after catheter ablation and may represent possible etiological factors for subsequent outcomes.
摘要：
导管消融术已被证明可以减少心房颤动（AF）的复发。导管消融术后房颤复发的机制尚不清楚，本研究旨在确定与房颤复发相关的血清蛋白。本前瞻性研究包括一组房颤患者，随访1年后分为两组：第1组包括导管消融术后发生代偿性房颤的患者，第2组包括导管消融术后发生房颤复发的患者。在分别从第1组和第2组招募的M1和M2小亚组中进行血清蛋白的初始微阵列分析。通过抗体微阵列来评估潜在的相关蛋白质。初始蛋白质组分析的数据鉴定了组1和组2中的候选蛋白质,然后通过ELISA测量它们的水平。分析的数据表明，M2亚组中RAD51和p63蛋白水平与M1亚组相比总体增加，表明这两种蛋白与房颤复发的潜在相关性。第1组和第2组的RAD51和p63水平的ELISA结果表明，与第1组相比，第2组（AF复发或基质AF）的RAD51（11.11±4.36vs8.45±4.85ng/mL；P=0.009）和p63（165.73±113.75vs标准化光密度100.05±37.56单位；P=0.0007）的水平增加（补偿）。因此，RAD51和p63与导管消融术后房颤复发相关，并可能代表后续结局的可能病因因素。
