PDF(Pubmed)
Abstract：
Akabane virus (AKAV) is an arbovirus belonging to the family Bunyaviridae, genus Orthobunyavirus. AKAV consists of three-segment (L, M, and S RNA segments), negative single-stranded RNA. The aim of this study was to investigate an in situ hybridization method (ISH) in a Vero E6 cell line infected with Akabane virus. The 320 base pair amplicon was obtained by RT-PCR with a primer pair and labeled with digoxigenin. Akabane virus RNAs were seen as a granular pattern in the cytoplasm of infected cells. As a result, the expression of the particular Akabane virus gene area was successfully disclosed in the current investigation using the ISH method with a digoxigenin-labeled probe.
摘要：
Akabane病毒（AKAV）是属于Bunyaviridae家族的虫媒病毒，直鼻病毒属。AKAV由三段组成(L，M,和SRNA片段)，负单链RNA。这项研究的目的是在感染Akabane病毒的VeroE6细胞系中研究原位杂交方法（ISH）。通过具有引物对的RT-PCR获得320个碱基对的扩增子,并用地高辛标记。Akabane病毒RNA在感染细胞的细胞质中被视为颗粒状模式。因此,在当前的研究中,使用具有地高辛标记的探针的ISH方法,成功地公开了特定的Akabane病毒基因区域的表达。
