关键词: 16S rRNA NGS bacterial community metagenomics microbial diversity polluted drains

来  源:   DOI:10.1002/jobm.202400303

Abstract:
Polluted drains across the globe are affected due to reckless disposal of untreated industrial effluents resulting in significant water pollution affecting microbial community structure/dynamics. To elucidate this, polluted samples were collected from Budha Nala (BN) drain, Tung Dhab (TD) drain, and wastewater treatment plant (WWTP) receiving an inflow of organic pollutants as well as heavy metals due to anthropogenic activities. The sample of unpolluted pristine soil (PS) was used as control, as there is no history of usage of organic chemicals at this site. The bacterial diversity of these samples was sequenced using the Illumina MiSeq platform by amplifying the V3/V4 region of 16S rRNA. The majority of operational taxonomic unit (OTUs) at polluted sites belonged to phyla Proteobacteria specifically Gammaproteobacteria class, followed by Actinobacteria, Bacteriodetes, Chloroflexi, Firmicutes, Planctomycetes, WS6, and TM7, whereas unpolluted site revealed the prevalence of Proteobacteria followed by Actinobacteria, Planctomycetes, Firmicutes, Acidobacteria, Chloroflexi, Bacteroidetes, Verrucomicrobia, and Nitrospirae. The data sets decode unclassified species of the phyla Proteobacteria, Bacteriodetes, Chloroflexi, Firmicutes, and WS6, along with some unclassified bacterial species. The study provided a comparative study of changed microbial community structure, their possible functions across diverse geographical locations, and identifying specific bacterial genera as pollution bio-indicators of aged polluted drains.
摘要:
由于不计后果地处置未经处理的工业废水,导致严重的水污染影响微生物群落结构/动态,全球污染的排水沟受到影响。为了阐明这一点,污染样品是从BudhaNala(BN)排水沟收集的,东达卜(TD)排水管,和污水处理厂(WWTP)由于人为活动而接收有机污染物以及重金属的流入。未污染的原始土壤(PS)样品用作对照,因为这里没有使用有机化学物质的历史。使用IlluminaMiSeq平台通过扩增16SrRNA的V3/V4区域对这些样品的细菌多样性进行测序。污染地点的大多数操作分类单位(OTU)属于门变形杆菌,特别是γ变形杆菌类,其次是放线菌,拟杆菌,氯氟菌,Firmicutes,Planctomycetes,WS6和TM7,而未污染的部位揭示了变形杆菌的流行,其次是放线菌,Planctomycetes,Firmicutes,酸杆菌,氯氟菌,拟杆菌,Verrucomicrobia,和Nitrosirae.数据集解码门变形杆菌的未分类物种,拟杆菌,氯氟菌,Firmicutes,和WS6以及一些未分类的细菌。该研究提供了变化的微生物群落结构的比较研究,它们在不同地理位置的可能功能,并确定特定的细菌属作为老化污染排水沟的污染生物指标。
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