PDF(Pubmed)
Abstract：
OBJECTIVE: Studies on the appropriate amount of anti-adhesive agents for preventing postoperative adhesion are lacking. This animal study aimed to investigate the distribution of an anti-adhesive agent in the abdominal cavity and estimate the necessary amount to cover the entire cavity.
METHODS: Fluorescent dye Flamma-552 was conjugated to Guardix-sol to create Guardix-Flamma, which was laparoscopically applied to the abdominal cavity of two 10-kg pigs in different amounts: 15 mL for G1 and 35 mL for G2. After 24 hours, the distribution of Guardix-Flamma was examined under the near-infrared mode of the laparoscope, and the thickness was measured in tissues from the omentum, small, and large intestine by immunohistochemistry.
RESULTS: The average area of the abdominal cavity in 10 kg pigs was 2,755 cm2. Guardix-Flamma fluorescence was detected in the greater omentum, ascites in the pelvis, and right quadrant area in G1, whereas in G2, it was detected everywhere. On average, the total thickness of G1 and G2 were 12.68 ± 9.80 μm and 18.16 ± 15.57 μm, respectively. Guardix-Flamma thickness applied to the omentum, small, and large intestines of G2 were 1.31-, 1.45-, and 1.49-times thicker than those of G1, respectively, and were all statistically significant (P < 0.05).
CONCLUSIONS: The entire abdominal cavity of the 10 kg pig was not evenly covered with 15 mL of Guardix. Although 35 mL of Guardix is sufficient to cover the same area with an average thickness of 18 µm, further studies should evaluate the minimum thickness required for an effective anti-adhesive function.
METHODS: Fluorescent dye Flamma-552 was conjugated to Guardix-sol to create Guardix-Flamma, which was laparoscopically applied to the abdominal cavity of two 10-kg pigs in different amounts: 15 mL for G1 and 35 mL for G2. After 24 hours, the distribution of Guardix-Flamma was examined under the near-infrared mode of the laparoscope, and the thickness was measured in tissues from the omentum, small, and large intestine by immunohistochemistry.
RESULTS: The average area of the abdominal cavity in 10 kg pigs was 2,755 cm2. Guardix-Flamma fluorescence was detected in the greater omentum, ascites in the pelvis, and right quadrant area in G1, whereas in G2, it was detected everywhere. On average, the total thickness of G1 and G2 were 12.68 ± 9.80 μm and 18.16 ± 15.57 μm, respectively. Guardix-Flamma thickness applied to the omentum, small, and large intestines of G2 were 1.31-, 1.45-, and 1.49-times thicker than those of G1, respectively, and were all statistically significant (P < 0.05).
CONCLUSIONS: The entire abdominal cavity of the 10 kg pig was not evenly covered with 15 mL of Guardix. Although 35 mL of Guardix is sufficient to cover the same area with an average thickness of 18 µm, further studies should evaluate the minimum thickness required for an effective anti-adhesive function.
摘要：
目的：目前尚缺乏预防术后粘连的适量抗粘连剂的研究。该动物研究旨在研究抗粘连剂在腹腔中的分布并估计覆盖整个腔所需的量。
方法：将荧光染料Flamma-552与Guardix-sol缀合以产生Guardix-Flamma，腹腔镜下应用于两只10千克猪的腹腔，数量不同：G1为15mL，G2为35mL。24小时后,在腹腔镜的近红外模式下检查了Guardix-Flamma的分布，从网膜组织中测量厚度，小,和大肠的免疫组织化学。
结果:10kg猪的腹腔平均面积为2,755cm2。在大网膜中检测到Guardix-Flamma荧光，骨盆的腹水,在G1中，右象限区域，而在G2中，到处都可以检测到。平均而言,G1和G2的总厚度为12.68±9.80μm和18.16±15.57μm，分别。适用于网膜的Guardix-Flamma厚度，小,G2的大肠为1.31-，1.45-，分别比G1厚1.49倍，均有统计学意义(P<0.05)。
结论：10kg猪的整个腹腔未被15mL的Guardix均匀覆盖。尽管35mL的Guardix足以覆盖平均厚度为18µm的相同区域，进一步的研究应该评估有效的抗粘连功能所需的最小厚度。
方法：将荧光染料Flamma-552与Guardix-sol缀合以产生Guardix-Flamma，腹腔镜下应用于两只10千克猪的腹腔，数量不同：G1为15mL，G2为35mL。24小时后,在腹腔镜的近红外模式下检查了Guardix-Flamma的分布，从网膜组织中测量厚度，小,和大肠的免疫组织化学。
结果:10kg猪的腹腔平均面积为2,755cm2。在大网膜中检测到Guardix-Flamma荧光，骨盆的腹水,在G1中，右象限区域，而在G2中，到处都可以检测到。平均而言,G1和G2的总厚度为12.68±9.80μm和18.16±15.57μm，分别。适用于网膜的Guardix-Flamma厚度，小,G2的大肠为1.31-，1.45-，分别比G1厚1.49倍，均有统计学意义(P<0.05)。
结论：10kg猪的整个腹腔未被15mL的Guardix均匀覆盖。尽管35mL的Guardix足以覆盖平均厚度为18µm的相同区域，进一步的研究应该评估有效的抗粘连功能所需的最小厚度。
