METHODS: The study aimed to assess the potential anti-cancer effects of Euphorbia extract on colon cancer cell lines (DLD-1) through various techniques. The apoptosis, migration, and proliferation of DLD-1 cells were measured in DLD-1 cells. In addition, we conducted High-Performance Liquid Chromatography (HPLC) analysis to identify the profile of phenolic compounds present in the studied extracts.
RESULTS: The extracts demonstrated inhibition of colon cancer cell migration. E. resinifera flower and E. echinus stem extracts show significant anti-migratory effects. Regarding anti-proliferative activity, E. resinifera flower extract hindered proliferation, whereas E. echinus flower extract exhibited dose-dependent inhibition. Apoptosis assays revealed E. resinifera flower extract inducing early-stage apoptosis and E. echinus flower extract promoting late-stage apoptosis. While apoptotic protein expression indicated, E. resinifera stem and propolis extracts had minimal impact on apoptosis.
CONCLUSIONS: The findings provide evidence supporting the beneficial effects of E resinifera and E. echinus extracts on colon cancer and exerting anti-cancer properties.
方法:该研究旨在通过各种技术评估一品红提取物对结肠癌细胞系(DLD-1)的潜在抗癌作用。细胞凋亡,迁移,在DLD-1细胞中测量DLD-1细胞的增殖。此外,我们进行了高效液相色谱(HPLC)分析,以确定所研究提取物中存在的酚类化合物的概况。
结果:提取物显示抑制结肠癌细胞迁移。E.resinifera花和E.chinus茎提取物显示出显著的抗迁移作用。关于抗增殖活性,E.resinifera花提取物阻碍增殖,而E.chinus花提取物表现出剂量依赖性抑制。细胞凋亡试验表明,E.resinifera花提取物诱导早期细胞凋亡,E.echinus花提取物促进晚期细胞凋亡。虽然凋亡蛋白表达表明,E.resinifera茎和蜂胶提取物对细胞凋亡的影响最小。
结论:这些发现提供了证据支持E.resinifera和E.echinus提取物对结肠癌和发挥抗癌特性的有益作用。