PDF(Pubmed)
Abstract：
BACKGROUND: Colon cancer, a prominent contributor to global cancer-related deaths, prompts the need for innovative treatment strategies. Euphorbia resinifera O. Berg (E. resinifera) and Euphorbia officinarum subsp. echinus Hook. f. & Coss Vindt (E. echinus) and their bee-derived products have been integral to traditional Moroccan medicine due to their potential health benefits. These plants have historical use in addressing various health issues, including cancer. However, their effects against colon cancer remain unclear, and the specific mechanisms underlying their anti-cancer effects lack comprehensive investigation.
METHODS: The study aimed to assess the potential anti-cancer effects of Euphorbia extract on colon cancer cell lines (DLD-1) through various techniques. The apoptosis, migration, and proliferation of DLD-1 cells were measured in DLD-1 cells. In addition, we conducted High-Performance Liquid Chromatography (HPLC) analysis to identify the profile of phenolic compounds present in the studied extracts.
RESULTS: The extracts demonstrated inhibition of colon cancer cell migration. E. resinifera flower and E. echinus stem extracts show significant anti-migratory effects. Regarding anti-proliferative activity, E. resinifera flower extract hindered proliferation, whereas E. echinus flower extract exhibited dose-dependent inhibition. Apoptosis assays revealed E. resinifera flower extract inducing early-stage apoptosis and E. echinus flower extract promoting late-stage apoptosis. While apoptotic protein expression indicated, E. resinifera stem and propolis extracts had minimal impact on apoptosis.
CONCLUSIONS: The findings provide evidence supporting the beneficial effects of E resinifera and E. echinus extracts on colon cancer and exerting anti-cancer properties.
METHODS: The study aimed to assess the potential anti-cancer effects of Euphorbia extract on colon cancer cell lines (DLD-1) through various techniques. The apoptosis, migration, and proliferation of DLD-1 cells were measured in DLD-1 cells. In addition, we conducted High-Performance Liquid Chromatography (HPLC) analysis to identify the profile of phenolic compounds present in the studied extracts.
RESULTS: The extracts demonstrated inhibition of colon cancer cell migration. E. resinifera flower and E. echinus stem extracts show significant anti-migratory effects. Regarding anti-proliferative activity, E. resinifera flower extract hindered proliferation, whereas E. echinus flower extract exhibited dose-dependent inhibition. Apoptosis assays revealed E. resinifera flower extract inducing early-stage apoptosis and E. echinus flower extract promoting late-stage apoptosis. While apoptotic protein expression indicated, E. resinifera stem and propolis extracts had minimal impact on apoptosis.
CONCLUSIONS: The findings provide evidence supporting the beneficial effects of E resinifera and E. echinus extracts on colon cancer and exerting anti-cancer properties.
摘要：
背景：结肠癌，全球癌症相关死亡的主要贡献者，提示需要创新的治疗策略。一品红O.Berg（E.树脂）和大白草亚种。echinusHook.f.&CossVindt(E.echinus）及其蜜蜂衍生产品因其潜在的健康益处而成为传统摩洛哥医学的组成部分。这些植物在解决各种健康问题方面具有历史用途，包括癌症.然而,它们对结肠癌的影响尚不清楚，其抗癌作用的具体机制缺乏全面的研究。
方法：该研究旨在通过各种技术评估一品红提取物对结肠癌细胞系（DLD-1）的潜在抗癌作用。细胞凋亡，迁移,在DLD-1细胞中测量DLD-1细胞的增殖。此外,我们进行了高效液相色谱（HPLC）分析，以确定所研究提取物中存在的酚类化合物的概况。
结果：提取物显示抑制结肠癌细胞迁移。E.resinifera花和E.chinus茎提取物显示出显著的抗迁移作用。关于抗增殖活性，E.resinifera花提取物阻碍增殖，而E.chinus花提取物表现出剂量依赖性抑制。细胞凋亡试验表明，E.resinifera花提取物诱导早期细胞凋亡，E.echinus花提取物促进晚期细胞凋亡。虽然凋亡蛋白表达表明，E.resinifera茎和蜂胶提取物对细胞凋亡的影响最小。
结论：这些发现提供了证据支持E.resinifera和E.echinus提取物对结肠癌和发挥抗癌特性的有益作用。
方法：该研究旨在通过各种技术评估一品红提取物对结肠癌细胞系（DLD-1）的潜在抗癌作用。细胞凋亡，迁移,在DLD-1细胞中测量DLD-1细胞的增殖。此外,我们进行了高效液相色谱（HPLC）分析，以确定所研究提取物中存在的酚类化合物的概况。
结果：提取物显示抑制结肠癌细胞迁移。E.resinifera花和E.chinus茎提取物显示出显著的抗迁移作用。关于抗增殖活性，E.resinifera花提取物阻碍增殖，而E.chinus花提取物表现出剂量依赖性抑制。细胞凋亡试验表明，E.resinifera花提取物诱导早期细胞凋亡，E.echinus花提取物促进晚期细胞凋亡。虽然凋亡蛋白表达表明，E.resinifera茎和蜂胶提取物对细胞凋亡的影响最小。
结论：这些发现提供了证据支持E.resinifera和E.echinus提取物对结肠癌和发挥抗癌特性的有益作用。
