PDF(Pubmed)
Abstract：
The search for prognostic markers in breast cancer has bumped into a typical feature of these tumors, intra and intertumoral heterogeneity. Changes in the expression profile, localization of these proteins or shedding to the surrounding stroma can be useful in the search for new markers. In this context, classification by molecular subtypes can bring perspectives for both diagnosis and screening for appropriate treatments. However, the Triple Negative (TN) subtype, which is already the one with the worst prognosis, lacks appropriate and consistent molecular markers. In this work, we analyzed 346 human breast cancer samples in tissue microarrays (TMA) from cases diagnosed with invasive breast carcinoma to assess the expression and localization pattern of Maspin and their correlation with clinical parameters. To complement our findings, we also used TCGA data to analyze the mRNA levels of these respective genes. Our data suggests that the TN subtype demonstrates a higher level of cytoplasmic Maspin compared to the other subtypes. Maspin transcript levels follow the same trend. However, TN patients with lower Maspin expression tend to have worse overall survival and free-survival metastasis rates. Finally, we used Maspin expression data to verify possible relationships with the clinicopathological information of our cohort. Our univariate analyses indicate that Maspin is related to the expression of estrogen receptor (ER) and progesterone receptor (PR). Furthermore, Maspin expression levels also showed correlation with Scarff-Bloom-Richardson (SBR) parameter, and stromal Maspin showed a relationship with lymph node involvement. Our data is not consistently robust enough to categorize Maspin as a prognostic marker. However, it does indicate a change in the expression profile within the TN subtype.
摘要：
在乳腺癌中寻找预后标志物已经成为这些肿瘤的典型特征，肿瘤内和肿瘤间异质性。表达谱的变化，这些蛋白质的定位或向周围基质的脱落可能有助于寻找新的标记。在这种情况下，按分子亚型分类可以为诊断和筛选适当的治疗方法带来前景。然而,三负（TN）亚型，这已经是预后最差的一个，缺乏适当和一致的分子标记。在这项工作中,我们分析了来自诊断为浸润性乳腺癌的病例的组织微阵列(TMA)中的346例乳腺癌样本,以评估Maspin的表达和定位模式及其与临床参数的相关性.为了补充我们的发现，我们还使用TCGA数据分析了这些基因的mRNA水平.我们的数据表明，与其他亚型相比，TN亚型显示出更高水平的细胞质Maspin。Maspin转录水平遵循相同的趋势。然而,具有较低Maspin表达的TN患者倾向于具有更差的总体生存率和自由生存率转移率。最后,我们使用Maspin表达数据来验证与我们队列的临床病理信息的可能关系.我们的单变量分析表明，Maspin与雌激素受体（ER）和孕激素受体（PR）的表达有关。此外，Maspin表达水平也显示与Scarff-Bloom-Richardson(SBR)参数相关,基质Maspin显示与淋巴结受累有关。我们的数据不够稳健，不足以将Maspin分类为预后标志物。然而,它确实表明TN亚型内表达谱的变化。
