Abstract：
Those involved in drug testing continue to grapple with the dynamic nature of emerging psychoactive substances (NPS) and their rapid infiltration into society. The challenge extends beyond merely detecting and measuring NPS using analytical tools; it also encompasses the complexities arising from the formation and presence of metabolites and degradation products. This study utilises liquid chromatography time-of-flight mass spectrometry to investigate the stability of new psychoactive substances in wastewater. Seven NPS compounds including 25C-NBOMe, 5F-APINACA 4-hydroxyphenyl, AB-PINACA, APINACA 4-hydroxyphenyl, fentanyl, norfentanyl and MDPV, along with their corresponding internal standard, were examined. Reference material for each NPS compound was introduced into a wastewater sample from a Wessex water treatment plant. The sample was then exposed to four different environments: room temperature, refrigerator temperature, acidification to pH 2, and the introduction of sodium metabisulfite. The findings highlight the critical dependence of storage conditions on target analytes, emphasizing the paramount importance of the time elapsed between collection and analysis for NPS wastewater analysis. Notably, synthetic cannabinoids exhibit limited stability in wastewater whereas cathinone-like substances demonstrate greater stability. Furthermore, metabolites prove to be more stable in wastewater than the parent drug, suggesting that focusing on metabolite detection may be more favourable for future analysis.
摘要：
那些参与药物测试的人继续努力应对新兴精神活性物质（NPS）的动态特性及其迅速渗透到社会中。挑战不仅限于使用分析工具检测和测量NPS;它还包括由代谢物和降解产物的形成和存在引起的复杂性。本研究利用液相色谱飞行时间质谱来研究废水中新型精神活性物质的稳定性。七个NPS化合物，包括25C-NBOMe，5F-APINACA4-羟基苯基，AB-PINACA,APINACA4-羟基苯基,芬太尼,降芬太尼和MDPV,以及相应的内部标准，进行了检查。将每种NPS化合物的参考材料引入到来自Wessex水处理厂的废水样品中。然后将样品暴露于四种不同的环境：室温，冰箱温度,酸化至pH2，并引入焦亚硫酸钠。研究结果强调了储存条件对目标分析物的关键依赖性，强调NPS废水分析收集和分析之间经过的时间至关重要。值得注意的是,合成大麻素在废水中表现出有限的稳定性，而卡西酮类物质表现出更高的稳定性。此外，代谢物在废水中被证明比母体药物更稳定，这表明专注于代谢物检测可能更有利于未来的分析。
