Abstract：
Experimental autoimmune encephalomyelitis (EAE) serves as a model for studying multiple sclerosis, with immunization strategies utilizing MOG35-55 peptide, emulsified in adjuvant enriched with mycobacterium tuberculosis (Mtb). This study examined the effects of Bacillus Calmette-Guérin (BCG) as an adjuvant, alongside the impact of MOG35-55 peptide doses and their residual counter ions on EAE development. We found that BCG can be effectively used to induce EAE with similar incidence and severity as heat-killed H37Ra, contingent upon the appropriate MOG35-55 peptide dose. Different immunization doses of MOG35-55 peptide significantly affect EAE development, with higher doses leading to a paradoxical reduction in disease activity, probably due to peripheral tolerance mechanisms. Furthermore, doses of MOG35-55 peptides with acetate showed a more pronounced effect on disease development compared to those containing trifluoroacetic acid (TFA), suggesting the potential influence of residual counter ions on EAE activity. We highlighted the feasibility of applying BCG to the establishment of EAE for the first time. Our findings emphasized the importance of MOG peptide dosage and composition in modulating EAE development, offering insights into the mechanisms of autoimmunity and tolerance. This could have implications for autoimmune disease research and the design of therapeutic strategies.
摘要：
实验性自身免疫性脑脊髓炎（EAE）作为研究多发性硬化症的模型，使用MOG35-55肽的免疫策略，在富含结核分枝杆菌（Mtb）的佐剂中乳化。这项研究检查了卡介苗（BCG）作为佐剂的作用，以及MOG35-55肽剂量及其残余抗衡离子对EAE发展的影响。我们发现BCG可以有效地用于诱导EAE，其发生率和严重程度与热灭活H37Ra相似，取决于适当的MOG35-55肽剂量。不同免疫剂量的MOG35-55肽显著影响EAE的发展,更高的剂量导致疾病活动的矛盾减少，可能是由于外周耐受机制。此外，与含有三氟乙酸（TFA）的那些相比，MOG35-55肽与乙酸盐的剂量对疾病发展显示出更明显的影响，表明残余抗衡离子对EAE活性的潜在影响。我们首次强调了将BCG应用于建立EAE的可行性。我们的发现强调了MOG肽剂量和组成在调节EAE发展中的重要性，提供对自身免疫和耐受机制的见解。这可能对自身免疫性疾病研究和治疗策略的设计产生影响。
