Abstract：
Henna is a plant-based dye obtained from the powdered leaf of the pigmented plant Lawsonia inermis, and has often been used for grey hair dyeing, treatment, and body painting. As a henna product, the leaves of Indigofera tinctoria and Cassia auriculata can be blended to produce different colour variations. Although allergy from henna products attributed to p-phenylenediamine, which is added to enhance the dye, is reported occasionally, raw material plants of henna products could also contribute to the allergy. In this study, we reported that raw material plants of commercial henna products distributed in Japan can be estimated by LC-high resolution MS (LC-HRMS) and multivariate analysis. Principal Component Analysis (PCA) score plot clearly separated 17 samples into three groups [I; henna, II; blended henna primarily comprising Indigofera tinctoria, III; Cassia auriculata]. This grouping was consistent with the ingredient lists of products except that one sample listed as henna was classified as Group III, indicating that its ingredient label may differ from the actual formulation. The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. This study is the first to estimate raw material plants used in commercial plant-based dye by LC-HRMS and multivariate analysis.
摘要：
指甲花是从色素植物Lawsoniainermis的粉末叶子中获得的植物性染料，并经常用于灰色头发染色，治疗,和身体绘画。作为指甲花产品，靛蓝和决明子的叶子可以混合以产生不同的颜色变化。虽然从指甲花产品归因于对苯二胺过敏，添加来增强染料，偶尔被报道，指甲花产品的原料植物也可能导致过敏。在这项研究中,我们报告说，在日本分布的商业指甲花产品的原料工厂可以通过LC-高分辨率MS（LC-HRMS）和多变量分析进行估算。主成分分析（PCA）得分图清楚地将17个样本分为三组[I；指甲花，II；混合指甲花主要包括靛蓝，III；决明子]。这一分组与产品的成分表一致，除了一个被列为指甲花的样本被归类为第三组，表明其成分标签可能与实际配方不同。第一组特有的成分，II,PCA的III是Lawsone(1)，靛玉红(2),芦丁（3），分别,据报道，它们作为成分包含在每种植物中。因此,指甲花产品可以被认为是从这些工厂制造的。这项研究是第一个通过LC-HRMS和多变量分析估计商业植物染料中使用的原料植物。
