Abstract：
The diagnosis of fungal infections presents several challenges and limitations, stemming from the similarities in symptomatology, diversity of underlying pathogenic species, complexity of fungal biology, and scarcity of rapid, affordable, and point-of-care approaches. In this review, we assess technological advances enabling the conversion of cutting-edge laboratory molecular diagnostic methods to cost-effective microfluidic devices. The most promising strategies toward the design of DNA sequence-based fungal diagnostic systems, capable of capturing and deciphering the highly informative DNA of the pathogen and adapted for resource-limited settings, are discussed, bridging fungal biology, molecular genetics, microfluidics, and biosensors.
摘要：
真菌感染的诊断存在一些挑战和局限性。源于症状学的相似性，潜在致病物种的多样性，真菌生物学的复杂性，和稀缺的快速，负担得起的,和即时护理方法。在这次审查中,我们评估了能够将尖端实验室分子诊断方法转化为具有成本效益的微流体设备的技术进步。基于DNA序列的真菌诊断系统的设计最有前途的策略，能够捕获和破译病原体的高度信息的DNA，并适应资源有限的环境，讨论，桥接真菌生物学，分子遗传学,微流体,和生物传感器。
