Abstract：
This study aimed to assess the effect of coating based on nanochitosan-whey protein isolate (NCH-WPI) containing summer savory essential oil (SEO) combined with oxygen absorber (OA) packaging on Pseudomonas aeruginosa, Listeria monocytogenes, and Escherichia coli O157H7, inoculated to rainbow trout fillets stored under refrigeration. Except control and OA groups, L. monocytogenes decreased (0.49-1.82 log CFU/g) in all treatment groups until the eighth day, and then increased (0.39-0.68 log CFU/g). This indicates that the treatments were ineffective to inhibit the proliferation of this bacterium. Considering the forced aerobic nature of inoculated P. aeruginosa, the counts of these bacteria become undetectable in groups packed with OA after the fourth day of storage, while the other groups showed an increase (0.99-2.23 log CFU/g) in this bacteria population during entire storage period. This growth rate was slower in the NCH-WPI + 1%SEO and NCH-WPI + 2%SEO groups. Regarding the inoculated E. coli, its count was decreased (1.48-2.41 log CFU/g) during storage, and this reduction (2.24-2.41 log CFU/g) was the highest in NCH-WPI + 1%SEO + OA and NCH-WPI + 2%SEO + OA groups. In conclusion, NCH-WPI treatments delayed the growth of all pathogenic bacteria, but the ternary treatment (NCH-WPI + SEO + OA) was the most effective treatment in this regard.
摘要：
本研究旨在评估基于含有夏季咸味精油（SEO）的纳米壳聚糖-乳清蛋白分离物（NCH-WPI）结合氧气吸收剂（OA）包装的涂层对铜绿假单胞菌的影响。单核细胞增生李斯特菌,和大肠杆菌O157H7，接种到冷藏储存的虹鳟鱼鱼片中。除对照组和OA组外，在所有治疗组中，单核细胞增生李斯特菌下降（0.49-1.82logCFU/g），直到第8天，然后增加（0.39-0.68logCFU/g）。这表明处理对于抑制该细菌的增殖是无效的。考虑到已接种铜绿假单胞菌的强制需氧性质，在储存第四天后，这些细菌的数量在充满OA的组中变得无法检测到，而其他组显示在整个存储期间该细菌种群的增加（0.99-2.23logCFU/g）。NCH-WPI+1%SEO和NCH-WPI+2%SEO组的增长率较慢。关于接种的大肠杆菌，其计数在储存期间减少(1.48-2.41logCFU/g)，在NCH-WPI+1%SEO+OA和NCH-WPI+2%SEO+OA组中，这一降低(2.24-2.41logCFU/g)是最高的。总之,NCH-WPI治疗延缓了所有病原菌的生长，但三元治疗(NCH-WPI+SEO+OA)是这方面最有效的治疗方法。
