Abstract：
Viral gastroenteritis is commonly reported in dogs and involves a great diversity of enteric viruses. In this research, viral diversity was investigated in dogs with diarrhea in Northern Brazil using shotgun metagenomics. Furthermore, the presence of norovirus (NoV) was investigated in 282 stool/rectal swabs of young/adult dogs with or without diarrhea from two public kennels, based on one-step reverse transcription polymerase chain reaction (RT-PCR) for genogroup VI and VII (GVI and GVII) and real-time RT-PCR for GI, GII, and GIV. Thirty-one viral families were identified, including bacteriophages. Phylogenetic analyses showed twelve complete or nearly complete genomes belonging to the species of Protoparvovirus carnivoran1, Mamastrovirus 5, Aichivirus A2, Alphacoronavirus 1, and Chipapillomavirus 1. This is the first description of the intestinal virome of dogs in Northern Brazil and the first detection of canine norovirus GVII in the country. These results are important for helping to understand the viral groups that circulate in the canine population.
摘要：
病毒性胃肠炎通常在狗中报道,并且涉及大量的肠道病毒。在这项研究中,在巴西北部使用猎枪宏基因组学研究了腹泻犬的病毒多样性。此外，对诺如病毒（NoV）的存在进行了调查，调查了282只年轻/成年犬的粪便/直肠拭子，无论是否有腹泻，来自两个公共犬舍，基于基因群VI和VII（GVI和GVII）的一步逆转录聚合酶链反应（RT-PCR）和GI的实时RT-PCR，GII,和GIV。确定了31个病毒家族，包括噬菌体.系统发育分析显示12个完整或几乎完整的基因组，属于食肉原病毒1，马胃病毒5，伊奇病毒A2，Alphacoronavirus1和Chipapillomavirus1。这是对巴西北部犬肠道病毒的首次描述，也是该国首次检测到犬诺如病毒GVII。这些结果对于帮助了解在犬科动物种群中传播的病毒组非常重要。
