PDF(Pubmed)
Abstract：
OBJECTIVE: Sensitization to staphylococcal superantigens (SAgs) could contribute to asthma severity. However, its relevance with eosinophilic phenotype has not yet been clarified. This study aimed to investigate associations between serum specific IgE levels to SAg and eosinophilic airway inflammation in adult asthmatics.
METHODS: The serum specific IgE levels to 3 SAgs, including staphylococcal enterotoxin A (SEA) and B (SEB), and toxic shock syndrome toxin-1 (TSST-1) were measured by ImmunoCAP in 230 adult asthmatic patients and 50 healthy controls (HCs). Clinical characteristics and laboratory parameters, including serum total/free IgE, and 2 eosinophil-activation markers, eosinophil cationic protein (ECP), and eosinophil-derived neurotoxin (EDN), were analyzed according to blood eosinophil counts (BEC; 150 cells/μL) and serum specific IgE levels to 3 SAgs (0.35 kU/L).
RESULTS: Asthmatic patients showed higher serum specific IgE levels to 3 SAgs than HCs (p < 0.05 for all). The serum total/clinfree IgE levels were significantly higher in asthmatics with positive IgE responses to 3 SAgs than those without (p < 0.05 for all). There were no significant differences in clinical parameters including age, asthma severity, comorbidities, or smoking according to IgE responses to 3 SAgs. Patients with positive IgE responses to SEB (not to SEA/TSST-1) had higher serum specific IgE levels to house dust mites and ECP/EDN as well as higher BEC with positive correlations between serum SEB-specific IgE levels and BEC/ECP/EDN (p < 0.05 for all).
CONCLUSIONS: These findings suggest that serum SEB-specific IgE levels could contribute to eosinophil activation as well as IgE production in adult asthma.
METHODS: The serum specific IgE levels to 3 SAgs, including staphylococcal enterotoxin A (SEA) and B (SEB), and toxic shock syndrome toxin-1 (TSST-1) were measured by ImmunoCAP in 230 adult asthmatic patients and 50 healthy controls (HCs). Clinical characteristics and laboratory parameters, including serum total/free IgE, and 2 eosinophil-activation markers, eosinophil cationic protein (ECP), and eosinophil-derived neurotoxin (EDN), were analyzed according to blood eosinophil counts (BEC; 150 cells/μL) and serum specific IgE levels to 3 SAgs (0.35 kU/L).
RESULTS: Asthmatic patients showed higher serum specific IgE levels to 3 SAgs than HCs (p < 0.05 for all). The serum total/clinfree IgE levels were significantly higher in asthmatics with positive IgE responses to 3 SAgs than those without (p < 0.05 for all). There were no significant differences in clinical parameters including age, asthma severity, comorbidities, or smoking according to IgE responses to 3 SAgs. Patients with positive IgE responses to SEB (not to SEA/TSST-1) had higher serum specific IgE levels to house dust mites and ECP/EDN as well as higher BEC with positive correlations between serum SEB-specific IgE levels and BEC/ECP/EDN (p < 0.05 for all).
CONCLUSIONS: These findings suggest that serum SEB-specific IgE levels could contribute to eosinophil activation as well as IgE production in adult asthma.
摘要：
目的：对葡萄球菌超抗原（SAgs）的敏感性可能与哮喘的严重程度有关。然而,其与嗜酸性粒细胞表型的相关性尚未明确.本研究旨在探讨成人哮喘患者血清SAg特异性IgE水平与嗜酸性粒细胞气道炎症之间的关系。
方法：血清特异性IgE水平对3个SAgs，包括葡萄球菌肠毒素A（SEA）和B（SEB），通过ImmunoCAP在230名成年哮喘患者和50名健康对照（HC）中测量了中毒性休克综合征毒素1（TSST-1）。临床特征和实验室参数，包括血清总/游离IgE，和2个嗜酸性粒细胞激活标记，嗜酸性粒细胞阳离子蛋白（ECP），和嗜酸性粒细胞衍生的神经毒素（EDN），根据血液嗜酸性粒细胞计数（BEC;150个细胞/μL）和血清特异性IgE水平对3个SAg（0.35kU/L）进行分析。
结果：哮喘患者对3个SAG的血清特异性IgE水平高于HC（全部p<0.05）。在对3个SAg有阳性IgE反应的哮喘患者中，血清总/非IgE水平显着高于没有的哮喘患者（全部p<0.05）。包括年龄在内的临床参数没有显着差异，哮喘严重程度，合并症,或根据IgE对3个SAG的反应吸烟。对SEB（非SEA/TSST-1）具有阳性IgE反应的患者对屋尘螨和ECP/EDN的血清特异性IgE水平较高，以及较高的BEC，血清SEB特异性IgE水平与BEC之间呈正相关。/ECP/EDN（全部p<0.05）。
结论：这些研究结果表明，血清SEB特异性IgE水平可能有助于成人哮喘中嗜酸性粒细胞活化和IgE的产生。
方法：血清特异性IgE水平对3个SAgs，包括葡萄球菌肠毒素A（SEA）和B（SEB），通过ImmunoCAP在230名成年哮喘患者和50名健康对照（HC）中测量了中毒性休克综合征毒素1（TSST-1）。临床特征和实验室参数，包括血清总/游离IgE，和2个嗜酸性粒细胞激活标记，嗜酸性粒细胞阳离子蛋白（ECP），和嗜酸性粒细胞衍生的神经毒素（EDN），根据血液嗜酸性粒细胞计数（BEC;150个细胞/μL）和血清特异性IgE水平对3个SAg（0.35kU/L）进行分析。
结果：哮喘患者对3个SAG的血清特异性IgE水平高于HC（全部p<0.05）。在对3个SAg有阳性IgE反应的哮喘患者中，血清总/非IgE水平显着高于没有的哮喘患者（全部p<0.05）。包括年龄在内的临床参数没有显着差异，哮喘严重程度，合并症,或根据IgE对3个SAG的反应吸烟。对SEB（非SEA/TSST-1）具有阳性IgE反应的患者对屋尘螨和ECP/EDN的血清特异性IgE水平较高，以及较高的BEC，血清SEB特异性IgE水平与BEC之间呈正相关。/ECP/EDN（全部p<0.05）。
结论：这些研究结果表明，血清SEB特异性IgE水平可能有助于成人哮喘中嗜酸性粒细胞活化和IgE的产生。
