Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) Virus.

禽流感病毒亚型 A （ H5 ） 的多重双目标逆转录 PCR 。

• 影响指数: 16.126
• 发表时间: 2024 Aug 10
• 来源期刊: Emerg Infect Dis
• DOI: 10.3201/eid3008.240785
• PMID: 38986151
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 医学
• 作者列表: Sahoo MK,Morante IEA,Huang C,Solis D,Yamamoto F,Ohiri UC,Romero D,Pinsky BA

关键词: H5; PCR; avian influenza; influenza; respiratory infections; subtyping; viruses; zoonoses

Mesh: Humans; Animals; Influenza in Birds / virology diagnosis; Influenza, Human / virology diagnosis; Reverse Transcriptase Polymerase Chain Reaction / methods; Influenza A Virus, H5N1 Subtype / genetics classification isolation & purification; Birds / virology; Multiplex Polymerase Chain Reaction / methods; Hemagglutinin Glycoproteins, Influenza Virus / genetics; Influenza A virus / genetics classification isolation & purification

Abstract

An increased risk for human infection with avian influenza A(H5N1) viruses is of concern. We developed an internally controlled, dual-target reverse transcription PCR for influenza A(H5) subtyping. This test could be used to detect influenza A(H5) in clinical samples.

摘要

人类感染甲型禽流感（H5N1）病毒的风险增加令人担忧。我们开发了一个内部控制的，甲型流感（H5）亚型的双靶逆转录PCR。该测试可用于检测临床样品中的流感A（H5）。