PDF(Pubmed)
Abstract：
Infectious and foodborne diseases pose significant global threats, with devastating consequences in low- and middle-income countries. Ozone, derived from atmospheric oxygen, exerts antimicrobial effects against various microorganisms, and degrades fungal toxins, which were initially recognized in the healthcare and food industries. However, highly concentrated ozone gas can be detrimental to human health. In addition, ozonated water is unstable and has a short half-life. Therefore, ultrafine-bubble technology is expected to overcome these issues. Ultrafine bubbles, which are nanoscale entitles that exist in water for considerable durations, have previously demonstrated bactericidal effects against various bacterial species, including antibiotic-resistant strains. This present study investigated the effects of ozone ultrafine bubble water (OUFBW) on various bacterial toxins. This study revealed that OUFBW treatment abolished the toxicity of pneumolysin, a pneumococcal pore-forming toxin, and leukotoxin, a toxin that causes leukocyte injury. Silver staining confirmed the degradation of pneumolysin, leukotoxin, and staphylococcal enterotoxin A, which are potent gastrointestinal toxins, following OUFB treatment. In addition, OUFBW treatment significantly inhibited NF-κB activation by Pam3CSK4, a synthetic triacylated lipopeptide that activates Toll-like receptor 2. Additionally, OUFBW exerted bactericidal activity against Staphylococcus aureus, including an antibiotic-resistant strain, without displaying significant toxicity toward human neutrophils or erythrocytes. These results suggest that OUFBW not only sterilizes bacteria but also degrades bacterial toxins.
摘要：
传染病和食源性疾病构成重大全球威胁，在低收入和中等收入国家造成毁灭性后果。臭氧,来自大气中的氧气，对各种微生物发挥抗菌作用，降解真菌毒素，最初在医疗保健和食品工业中得到认可。然而,高浓度的臭氧气体可能对人体健康有害。此外,臭氧水不稳定，半衰期短。因此,超细气泡技术有望克服这些问题。超微气泡,它们是在水中存在相当长的时间的纳米级权利，以前已经证明了对各种细菌的杀菌作用，包括抗生素抗性菌株。本研究调查了臭氧超细气泡水（OUFBW）对各种细菌毒素的影响。这项研究表明，OUFBW治疗消除了肺炎球菌溶血素的毒性，一种肺炎球菌成孔毒素,和白细胞毒素,引起白细胞损伤的毒素。银染证实了肺炎球菌溶血素的降解，白细胞毒素,和葡萄球菌肠毒素A,是强效的胃肠毒素，在OUFB治疗之后。此外,OUFBW处理通过Pam3CSK4显著抑制NF-κB激活,Pam3CSK4是一种激活Toll样受体2的合成三酰化脂肽。此外,OUFBW对金黄色葡萄球菌具有杀菌活性，包括抗生素抗性菌株，对人类中性粒细胞或红细胞无明显毒性。这些结果表明,OUFBW不仅灭菌细菌而且降解细菌毒素。
