Abstract：
Epidemiology of shigellosis has drastically changed in recent years due to globalization and sexual risk behaviors. Here, through whole-genome sequencing, we characterized two ESBL-producing Shigella sonnei strains (ShSoBUH1 and ShSoBUH2) carrying a blaCTX-M-15 among men who have sex with men (MSM), who had not recently traveled and presented sexual risk behaviors. Both strains harbored IncB/O/K/Z and IncFII plasmids, which carry aadA1, aadA5, sul1, sul2, dfrA1, dfrA17, mph(A), erm(B), tet(B), qacE and blaCTX-M-15 genes conferring resistance to 2nd and 3rd generation cephalosporins, cotrimoxazole, erythromycin, azithromycin and quinolones. IncFII plasmids containing blaCTX-M-15 from ShSoBUH1 and ShSoBUH2 presented 99,8-99,9% similarity with plasmids from another five CTX-M-15 S. sonnei strains detected in Belgium and Switzerland. A single-nucleotide polymorphism (SNP) analysis determined that the study strains differed by 361 SNPs, belonging to different clusters. To the best of our knowledge, this is the first report describing two extensively drug-resistant (XDR) CTX-M-15 S. sonnei strains in MSM.
摘要：
近年来，由于全球化和性危险行为，志贺氏菌病的流行病学发生了巨大变化。这里,通过全基因组测序，我们鉴定了在男男性行为者（MSM）中携带blaCTX-M-15的两种产ESBL志贺氏菌（ShSoBUH1和ShSoBUH2），最近没有旅行并表现出性行为的人。两种菌株都含有IncB/O/K/Z和IncFII质粒，携带aadA1，aadA5，sul1，sul2，dfrA1，dfrA17，mph（A），erm(B)，tet(B),qacE和blaCTX-M-15基因赋予对第二代和第三代头孢菌素的抗性，复方新诺明,红霉素,阿奇霉素和喹诺酮类药物。含有来自ShSoBUH1和ShSoBUH2的blaCTX-M-15的IncFII质粒与来自比利时和瑞士检测到的另外五个CTX-M-15S.sonnei菌株的质粒具有99,8-99,9％的相似性。单核苷酸多态性（SNP）分析确定研究菌株有361个SNP，属于不同的集群。据我们所知,这是第一份报告描述MSM中两种广泛耐药（XDR）的CTX-M-15S.sonnei菌株。
