Abstract：
OBJECTIVE: To determine whether there is a significant association between inflammatory cytokines in the tear fluid and the severity of Retinopathy of Prematurity (ROP).
METHODS: Retrospective cohort study.
METHODS: The cytokine levels in tear fluids were determined in 34 eyes with ROP and 18 eyes without ROP. There were 15 eyes with severe ROP requiring treatment and 19 eyes with mild ROP not requiring treatment. For severe ROP eyes, tear fluids were collected before treatment.
RESULTS: Significantly higher levels of CCL2 and vascular endothelial growth factor (VEGF) were detected in eyes with severe ROP compared to eyes with mild ROP and no ROP. When assessed for cytokine levels that discriminate each disease group, CCL2 showed a significant odds ratio of 1.76 for severity change (/quintile, P = 0.032, after adjusting for birth weight). Correlation analysis showed that birth weight correlated with IL-1α levels, and decreased weight gain increased IFN-γ levels. We next determined tear fluid cytokines which discriminate severe ROP using receiver operating characteristics\' analysis. We found that combination of higher CCL2 levels, higher VEGF levels, and lower IFN-γ levels in the tear fluid had a stronger predictive value for severe ROP (area under curve, 0.85).
CONCLUSIONS: The levels of CCL2, VEGF, and IFN-γ in tear fluid may serve as useful biomarkers for assessing the severity of ROP.
METHODS: Retrospective cohort study.
METHODS: The cytokine levels in tear fluids were determined in 34 eyes with ROP and 18 eyes without ROP. There were 15 eyes with severe ROP requiring treatment and 19 eyes with mild ROP not requiring treatment. For severe ROP eyes, tear fluids were collected before treatment.
RESULTS: Significantly higher levels of CCL2 and vascular endothelial growth factor (VEGF) were detected in eyes with severe ROP compared to eyes with mild ROP and no ROP. When assessed for cytokine levels that discriminate each disease group, CCL2 showed a significant odds ratio of 1.76 for severity change (/quintile, P = 0.032, after adjusting for birth weight). Correlation analysis showed that birth weight correlated with IL-1α levels, and decreased weight gain increased IFN-γ levels. We next determined tear fluid cytokines which discriminate severe ROP using receiver operating characteristics\' analysis. We found that combination of higher CCL2 levels, higher VEGF levels, and lower IFN-γ levels in the tear fluid had a stronger predictive value for severe ROP (area under curve, 0.85).
CONCLUSIONS: The levels of CCL2, VEGF, and IFN-γ in tear fluid may serve as useful biomarkers for assessing the severity of ROP.
摘要：
目的：确定泪液中的炎性细胞因子与早产儿视网膜病变（ROP）的严重程度之间是否存在显着关联。
方法：回顾性队列研究。
方法：测定34只眼ROP和18只眼无ROP的泪液中细胞因子水平。有15只眼患有重度ROP需要治疗,19只眼患有轻度ROP不需要治疗。对于严重的ROP眼睛，治疗前收集泪液。
结果：与轻度ROP和无ROP的眼睛相比，重度ROP的眼睛中检测到的CCL2和血管内皮生长因子（VEGF）水平明显更高。当评估区分每个疾病组的细胞因子水平时，CCL2显示严重程度变化的显著比值比为1.76(/五分位数，P=0.032，调整出生体重后）。相关分析显示,出生体重与IL-1α水平相关,和减少体重增加增加IFN-γ水平。我们接下来使用接受者操作特征分析来确定区分严重ROP的泪液细胞因子。我们发现更高的CCL2水平的组合，更高的VEGF水平，和较低的IFN-γ水平在泪液中对严重ROP有更强的预测价值（曲线下面积，0.85)。
结论：CCL2、VEGF、泪液中的IFN-γ和IFN-γ可作为评估ROP严重程度的有用生物标志物。
方法：回顾性队列研究。
方法：测定34只眼ROP和18只眼无ROP的泪液中细胞因子水平。有15只眼患有重度ROP需要治疗,19只眼患有轻度ROP不需要治疗。对于严重的ROP眼睛，治疗前收集泪液。
结果：与轻度ROP和无ROP的眼睛相比，重度ROP的眼睛中检测到的CCL2和血管内皮生长因子（VEGF）水平明显更高。当评估区分每个疾病组的细胞因子水平时，CCL2显示严重程度变化的显著比值比为1.76(/五分位数，P=0.032，调整出生体重后）。相关分析显示,出生体重与IL-1α水平相关,和减少体重增加增加IFN-γ水平。我们接下来使用接受者操作特征分析来确定区分严重ROP的泪液细胞因子。我们发现更高的CCL2水平的组合，更高的VEGF水平，和较低的IFN-γ水平在泪液中对严重ROP有更强的预测价值（曲线下面积，0.85)。
结论：CCL2、VEGF、泪液中的IFN-γ和IFN-γ可作为评估ROP严重程度的有用生物标志物。
