Abstract：
Adhesive dentistry has made it feasible to design restorations with high aesthetic qualities and little invasiveness. However, the freshly cut dentin after the tooth preparation needs to be sealed to prevent its contaminations, bacterial leakage, and hypersensitivity. Consequently, the immediate dentin sealing (IDS) method has been advised. This study examines different dentinal tubule sealing methods via CO2 laser, diode laser (980-nm) and a two-step self-etch adhesive system applied directly to the fresh cut dentin preceding the placement of the provisional phase. The sealing efficiency of each laser and bond system was evaluated based on the laser-induced fluorescence (LIF) properties and image analysis by scanning electron microscopy. Moreover, the obtained LIF spectra were evaluated using partial least square progression. A two-step adhesive containing a high concentration of S-PRG fillers produced a thick layer that was not perfectly uniform at all sites due to uneven filler distribution in the bond with totally and partially closed dentinal tubules. However, the peaks of the LIF spectra dropped after applying laser because of its sealing effectiveness. Accordingly, CO2 and diode lasers have strong evidence in dentinal tubule sealing and a definitive treatment modality for dentinal hypersensitivity. Moreover, IDS with an adhesive system is superior in occluding dentinal tubules in a biomimetic manner based on its filler content and bioactive properties.
摘要：
粘附性牙科使设计具有高美学品质和小侵入性的修复体变得可行。然而,牙齿准备后新鲜切割的牙本质需要密封以防止其污染，细菌渗漏,和超敏反应。因此,建议立即密封牙本质（IDS）方法。本研究通过CO2激光检查了不同的牙本质小管密封方法，二极管激光器（980nm）和两步自蚀刻粘合剂系统，在放置临时阶段之前直接应用于新鲜切割的牙本质。基于激光诱导荧光（LIF）特性和扫描电子显微镜的图像分析，评估了每种激光和粘合系统的密封效率。此外，使用偏最小二乘级数评价获得的LIF光谱。含有高浓度S-PRG填料的两步粘合剂产生的厚层在所有部位都不完全均匀，这是由于填料在与完全和部分封闭的牙本质小管的结合中分布不均匀。然而,LIF光谱的峰值在应用激光后由于其密封效果而下降。因此，CO2和二极管激光在牙本质小管密封和牙本质超敏反应的明确治疗方式方面具有强有力的证据。此外，基于其填充物含量和生物活性特性，具有粘附系统的IDS在以仿生方式闭塞牙本质小管方面具有优势。
