Abstract：
Cryptosporidium infection is a common occurrence in rodents worldwide. In this study, 435 wild brown rats were captured from an animal feedlot in Xinjiang, China, with a fecal sample obtained directly from the rectal contents of each rat. The DNA extracted from these fecal samples was analyzed for Cryptosporidium spp. using PCR targeting the SSU rRNA gene. The prevalence of Cryptosporidium infection in brown rats was found to be 5.5% (24 out of 435). Interestingly, the infection rates varied among different animal enclosures, with rates of 0% in the chicken coop (0/51), cowshed (0/3), and varying rates in other areas including the sheepfold (6.1%, 6/98), the pigsty (7.6%, 10/132), the dovecote (7.0%, 5/71), and outdoor environments (3.8%, 3/80). The study identified three species and one genotype of Cryptosporidium, namely C. occultus (n = 10), C. parvum (n = 4), C. ditrichi (n = 1), and Cryptosporidium rat genotype IV (n = 9). Additionally, two of the C. parvum isolates were successfully subtyped as IIdA19G1 (n = 2) at the gp60 gene. These results offer valuable insights into the prevalence and genetic diversity of Cryptosporidium in brown rats within the region.
摘要：
隐孢子虫感染在世界范围内的啮齿动物中很常见。在这项研究中,新疆某动物饲养场捕获野生褐鼠435只,中国,直接从每只大鼠的直肠内容物中获得粪便样本。分析了从这些粪便样品中提取的DNA的隐孢子虫。使用PCR靶向SSUrRNA基因。发现棕色大鼠中隐孢子虫感染的患病率为5.5％（435只中有24只）。有趣的是,不同动物围栏的感染率不同，鸡舍率为0%(0/51)，牛棚（0/3），以及包括羊圈在内的其他地区的不同比率(6.1%，6/98),猪圈(7.6%，10/132),dovecote(7.0%，5/71),和户外环境(3.8%，3/80）。该研究确定了隐孢子虫的三种和一种基因型，即C.occultus(n=10)，C.parvum(n=4)，C.ditrichi(n=1)，和隐孢子虫大鼠基因型IV（n=9）。此外,在gp60基因上，成功地将两个C.parvum分离株亚型为IIdA19G1（n=2）。这些结果为该地区棕色大鼠隐孢子虫的患病率和遗传多样性提供了有价值的见解。
